MAP Kinase Signaling Protocols

Activation and Function of Components of the MAP Kinase Signaling Cascades.- The MAP Kinase Signaling Cascades: A System of Hundreds of Components Regulates a Diverse Array of Physiological Functions.- Determination of ERK Activity: Anti-phospho-ERK Antibodies and In Vitro Phosphorylation.- Activation of SAPK/JNKs In Vitro.- Activation of p38 and Determination of Its Activity.- Activity Assays for Extracellular Signal-Regulated Kinase 5.- Use of Inhibitors in the Study of MAP Kinases.- Study of MAP Kinase Cascades as Transmitters of Membranal Receptor Signals.- MAP Kinase Activation by Receptor Tyrosine Kinases: In Control of Cell Migration.- Activation of Ras and Rho GTPases and MAP Kinases by G-Protein-Coupled Receptors.- Regulation of MAP Kinase Signaling by Calcium.- Identification of Novel Substrates of MAP Kinase Cascades Using Bioengineered Kinases that Uniquely Utilize Analogs of ATP to Phosphorylate Substrates.- ERK-MAP Kinase Signaling in the Cytoplasm.- Lentiviral Vectors to Study the Differential Function of ERK1 and ERK2 MAP Kinases.- Structure-Function Relationships and Localization of MAP Kinases.- Structural Studies of MAP Kinase Cascade Components.- Analysis of MAP Kinases by Hydrogen Exchange Mass Spectrometry.- A “Molecular Evolution” Approach for Isolation of Intrinsically Active (MEK-Independent) MAP Kinases.- Reconstitution of the Nuclear Transport of the MAP Kinase ERK2.- Localization and Trafficking of Fluorescently Tagged ERK1 and ERK2.- Studies on the Regulation of MAP Kinase Cascades.- Studying the Regulation of MAP Kinase by MAP Kinase Phosphatases In Vitro and in Cell Systems.- Proteomic Analysis of Scaffold Proteins in the ERK Cascade.- Analysis of ERKs’ Dimerization by Electrophoresis.- MAP Kinase: SUMO Pathway Interactions.-Computational Modelling of Kinase Signalling Cascades.- Use of Lower Organisms, Animal Models, and Human Genetics in the Study of MAP Kinases.- Analysis of Mitogen-Activated Protein Kinase Activity in Yeast.- Detection of RTK Pathway Activation in Drosophila Using Anti-dpERK Immunofluorescence Staining.- Studying MAP Kinase Pathways During Early Development of Xenopus laevis.- Deciphering Signaling Pathways In Vivo: The Ras/Raf/Mek/Erk Cascade.- Mutational and Functional Analysis in Human Ras/MAP Kinase Genetic Syndromes.- Study of MAP Kinases in Specific Physiological Systems.- Implication of the ERK Pathway on the Post-transcriptional Regulation of VEGF mRNA Stability.- Studies on MAP Kinase Signaling in the Immune System.- Methods to Study MAP Kinase Signalling in the Central Nervous System.- MAP Kinase Regulation of the Mitotic Spindle Checkpoint.- Using High-Content Microscopy to Study Gonadotrophin-Releasing Hormone Regulation of ERK.