Diagnosis of Sexually Transmitted Diseases

Overview of Molecular Biological Methods for the Detection of Pathogens Causing Sexually Transmitted Infections.- Guidelines for the Qualitative Detection of Viral Genomes in Dried Blood Spots.- Guidelines for the Quantification of HIV and HCV in Small Volume Whole Blood Samples.- Guidelines for High-Resolution Genotyping of Chlamydia trachomatis Using Multilocus Sequence Analysis.- Guidelines for the Use of Molecular Tests for the Detection and Genotyping of Human Papilloma Virus from Clinical Specimens.- The Molecular Diagnosis of Sexually Transmitted Genital Ulcer Disease.- Validation of a Sensitive and Specific Real-Time PCR for Detection and Quantitation of Hepatitis B Virus Covalently Closed Circular DNA in Plasma of Chronic Hepatitis B Patients.- Protocol for the Detection of Mycoplasma genitalium by PCR from Clinical Specimens and Subsequent Detection of Macrolide Resistance Mediating Mutations in Region V of the 23S rRNA Gene.- Protocols for Detection and Typing of Treponema pallidum Using PCR Methods.- Protocol for the Extraction of DNA from Dried Blood in the Diagnosis of Convenital CMV Infection.- Protocol for the Use of PCR-Denaturing Gradient Gel Electrophoresis and Quantitative PCR to Determine Vaginal Microflora Constitution and Pathogens in Bacterial Vaginosis.- Protocol for the Use of a Bead Array for the Multiple Detection of Genotype of Chlamydia trachomatis.- Protocol for the Detection and Genotyping of Human Papilloma viruses Using a Liquid Bead Microarray Assay (LBMA).- Protocol for the Use of Enzyme-Linked Hybridisation Assays for Genital Ulcer Disease.- Protocol for the Rapid Detection of the Urogential Tract Mollicutes and Chlamydia with Concomitant LGV –(sub)typing.- Protocol for the Use of Self-Reporting Duplex Mutation Primers to Detect PCR Products in the Diagnosis of HBV.- Protocol for the Use of a Rapid Real-Time PCR Method for the Detection of HIV-1 Proviral DNA Using Double-Stranded Primer.- Protocol for the Use of Light Upon Extension (LUX) Real-Time PCR for the Determination of Viral Load in HBV Infection.- Protocol for the Use of a Silica Nanoparticle-Enhanced Microcantilever Sensor-Based Method to Detect HBV at Femtomolar Concentrations.- Protocol for the Detection of Treponema pallidum in Paraffin-Embedded Specimens.- Guidelines for the Use of Molecular Biological Methods to Detect Sexually Transmitted Pathogens in Cases of Suspected Sexual Abuse in Children.- Protocol for the Molecular Detection of Antibiotic Resistance Mechanisms in Neisseria gonorrhoeae.- Protocol for a Facile Multiplex PCR for Multi-Antimicrobial Resistance and Gonococcus Detection.- Protocol for Gene Expression Profiling Using DNA Microarrays in Neisseria gonorrhoeae.- Protocol for the Detection of Human Leukocyte Antigen Variants and Sexually Transmitted Infections: From Genotyping to Immunoassays.- Protocol for the Clonal Analysis of NK Cell Effector Functions by Multi-Parameter Flow Cytometry.- Protocol for a Mammalian Cell-Based Assay for Monitoring the HIV-1 Protease Activity.- Self Collection of Specimens for Nucleic Acid-Based Diagnosis of Pharyngeal, Cevicovaginal, Urethral and Rectal Neisseria gonorrhoeae and Chlamydia trachomatisInfections.- Sexually Transmitted Diseases – Reflections on Metaphors and Ethics.