Electron Crystallography of Soluble and Membrane Proteins

Introduction to Electron Crystallography.- Practical Aspects in Expression and Purification of Membrane Proteins for Structural Analysis.- Two-Dimensional Crystallization of Membrane Proteins by Reconstitution through Dialysis.- Monolayer Two-Dimensional Crystallization.- Screening for Two-Dimensional Crystals by Transmission Electron Microscopy of Negatively Stained Samples.- Low Dose Techniques and Cryo-Electron Microscopy.- Grid Preparation for Cryo-Electron Microscopy.- Recording High-Resolution Images of Two-Dimensional Crystals of Membrane Proteins.- The Collection of High-Resolution Electron Diffraction Data.- Image Processing of 2D Crystal Images.- Merging of Image Data in Electron Crystallography.- Evaluation of Electron Crystallographic Data From Images of Two-Dimensional Crystals.- Modeling, Docking, and Fitting of Atomic Structures to 3D Maps from Cryo-Electron Microscopy.- Phasing Electron Diffraction Data by Molecular Replacement: Strategy for Structure Determination and Refinement.- High-Throughput Methods for Electron Crystallography.- Automated Grid Handling and Image Acquisition for Two-Dimensional Crystal Screening.- Automation of Data Acquisition in Electron Crystallography.- Automation of Image Processing in Electron Crystallography.- Choice and Maintenance of Equipment for Electron Crystallography.- Future Developments in Instrumentation for Electron Crystallography.- Tubular Crystals and Helical Arrays: Structural Determination of HIV-1 Capsid Assemblies Using Iterative Helical Real-Space Reconstruction.- Single Particle Electron Microscopy.- Electron Tomography of Paracrystalline 2D Arrays.- High-Resolution Imaging of 2D Outer Membrane Protein F (OmpF) Crystals by Atomic Force Microscopy.- Determination of Soluble and Membrane Protein Structures by X-Ray Crystallography.- Solution Nuclear Magnetic Resonance (NMR) Spectroscopy.- Structure-Function Insights of Membrane and Soluble Proteins Revealed by Electron Crystallography.- Lipid Monolayer and Sparse Matrix Screening for Growing Two-Dimensional Crystals for Electron Crystallography: Methods and Examples.- Processing of Electron Diffraction Patterns with the XDP Program.- Future Directions of Electron Crystallography.