Human Pluripotent Stem Cells

The Stem Cell Laboratory: Design, Equipment, and Oversight.- Stem Cell Banks: Preserving Cell Lines, Maintaining Genetic Integrity, and Advancing Research.- Embryonic Stem Cell Derivation from Human Embryos.- Derivation of Human Parthenogenetic Stem Cell Lines.- Generation of Induced Pluripotent Stem Cell Lines from Human Fibroblasts via Retroviral Gene Transfer.- Derivation of Induced Pluripotent Stem Cells by Lentiviral Transduction.- Transgene-free Production of Pluripotent Stem Cells using PiggyBac Transposons.- Traditional Human Embryonic Stem Cell Culture.- Xeno-Free Culture of Human Pluripotent Stem Cells.- Adaptation of Human Pluripotent Stem Cells to Feeder-Free Conditions in Chemically-Defined Medium with Enzymatic Single-Cell Passaging.- GMP Scale-Up and Banking of Pluripotent Stem Cells for Cellular Therapy Applications.- Culture of Human Pluripotent Stem Cells on Glass Slides for High-Resolution Imaging.- Classical Cytogenetics: Karyotyping Techniques.- FISH Analysis of Human Pluripotent Stem Cells.- Immunocytochemical Analysis of Human Pluripotent Stem Cells.- Flow Cytometric Analysis of Human Pluripotent Stem Cells.- The Teratoma Assay: An In vivo Assessment of Pluripotency.- Detection of Copy Number Variation using SNP Genotyping.- Genome-wide Epigenetic Analysis of Human Pluripotent Stem Cells by ChIP and ChIP-Seq.- Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays.- Development of High Content Screening Approaches and Analysis for Human Pluripotent Stem Cells.- Quantitative Proteome and Phosphoproteome Analysis of Human Pluripotent Stem Cells.- Lentivirus-mediated Modification of Pluripotent Stem Cells.- Nucleofection of Human Embryonic Stem Cells.- Non-viral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells.- Gene Targeting in Human Pluripotent Stem Cells.- Episomal Transgene Expression in Pluripotent Stem Cells.- The Generation of Embryoid Bodies from Feeder-based or Feeder-free Human Pluripotent Stem Cell Cultures.- Derivation of Oligodendrocyte Progenitor Cells from Human Embryonic Stem Cells.- Directed Differentiation of Dopamine Neurons From Human Pluripotent Stem Cells.- Methods for the Derivation and Use of Cardiomyocytes from Human Pluripotent Stem Cells.- In Vivo Evaluation of Putative Hematopoietic Stem Cells Derived from Human Pluripotent Stem Cells.- Differentiation of Dendritic Cells from Human Embryonic Stem Cells.