Plant Membrane Proteomics

Free Flow Zonal Electrophoresis for Fractionation of Plant Membrane Compartments prior to Proteomic Analysis.- Isolation and Purity Assessment of Membranes from Norway Spruce.- Nuclear Proteome: Isolation of Intact Nuclei, Extraction of Nuclear Proteins and 2-DE Analysis.- Identification of Plant Nuclear Proteins Based on a Combination of Flow Sorting, SDS-PAGE, and LC-MS/MS Analysis.- Isolation, Purity Assessment, and Proteomic Analysis of Nuclei.- Proteomic Analysis of Rice Golgi Membranes Isolated by Floating through Discontinuous Sucrose Density Gradient.- Analyzing the Vacuolar Membrane (Tonoplast) Proteome.- Preparation of Membrane Fractions (Envelope, Thylakoids, Grana and Stroma Lamellae) from Arabidopsis Chloroplasts for Quantitative Proteomic Investigations and Other Studies.- Isolation of Intact Thylakoid Membranes from Heterocytes of Filamentous, Nitrogen-Fixing Cyanobacteria.- Targeted Quantification of Isoforms of a Thylakoid-Bound Protein: MRM Method Development.- Sample Preparation for Analysis of the Plant Mitochondrial membrane Proteome.- Plasma Membrane Proteomics of Arabidopsis Suspension Cultured Cells Associated with Growth Phase using Nano-LC-MS/MS.- Mini-Scale Isolation and Preparation of Plasma Membrane Proteins from Potato Roots for LC/MS Analysis.- Assay of Plasma Membrane H+-ATPase in Plant Tissues under Abiotic Stresses.- Absolute Quantification of In Vitro Expressed Plant Membrane Proteins by Targeted Proteomics (MRM) for the Determination of Kinetic Parameters.- MSE for Label-Free Absolute Protein Quantification in Complex Proteomes.- Identification and Characterization of Plant Membrane Proteins using ARAMEMNON.- VANTED: A Tool for Integrative Visualization and Analysis of –Omics Data.