Dynamics of Membrane Proteins and Cellular Energetics

One : Theoretical Introduction.- The structure and function of Ca+2 - and phospholipid- dependent protein kinase (protein kinase C), a trans-membrane signal transducer.- Biosynthesis and mode of insertion of a stalked intrinsic membrane protein of the small intestinal brush border.- Tools and approaches to study membranes and cell organelles biogenesis.- Fluid-phase endocytosis and lysosomal enzyme excretion two facets of intracellular membrane traffic.- The neutrophil leukocyte. Properties and mechanism of activation.- Monoclonal antibodies for immunoanalysis.- Two : Experimental Section.- Experiment n° 1 : Trans-membrane signaling via protein kinase C and its inhibition by staurosporine.- Experiment n° 2 : Endocytosis analysis by flow cytofluorometry : study of the endocytosis of fluoresceinylated neoglycoproteins via membrane lectins of tumor cells.- Experiment n° 3 : Neutrophils. Respiratory burst and exocytosis.- Experiment n°4 : Endocytosis and exocytosis in Dictyostelium amoebae.- Experiment n° 5 : Isolation of intact chloroplasts - Criteria of integrity.- Experiment n° 6 : Methods for the isolation of higher plant vacuoles.- Experiment n° 7 : Assay of in vitro microtubule assembly. Effect of microtubule associated proteins.- Experiment n° 8 : Histamine release from human basophiles.- Experiment n° 9 : Intracellular Ca2+ measurements in intact neutrophils.- Experiment n° 10 : Synthesis and maturation of D-?-hydroxybutyrate dehydrogenase (BDH) from mitochondrial inner membrane.- Experiment n° 11 : Electropermeabilization and electrofusion of cells.- Experiment n° 12 : Use of monoclonal antibody against keratin in immunocytochemical techniques.