Bacteriophages
Humana Press Inc. (Verlag)
978-1-4939-7341-5 (ISBN)
Cutting edge and authoritative, Bacteriophages: Methods and Protocols, Volume III is a valuable resource for both established and novice phage scientists.
Basic Phage Mathematics.- Analysis of Host-Takeover during SPOi Infection of Bacillus subtilis.- Practical Advice on the One-Step Growth Curve.- Iron Chloride Flocculation of Bacteriophages from Seawater.- Purification of Bacteriophages using Anion-Exchange Chromatography.- Encapsulation Strategies of Bacteriophages (Felix O1) for Oral Therapeutic Application.- Encapsulation of Listeria Phage A511 by Alginate to Improve its Thermal Stability.- Application of a Virucidal Agent to Avoid Over-Estimation of Phage Kill during Phage Decontamination Assays on Ready-to-Eat Meats.- Sequencing, Assembling, and Finishing Complete Bacteriophage Genomes.- Identification of DNA Base Modifications by Means of Pacific Biosciences RS Sequencing Technology.- Analyzing Genome Termini of Bacteriophage Through High-Throughput Sequencing.- Amplification for Whole Genome Sequencing of Bacteriophages from Single Isolated Plaques using SISPA.- Genome Sequencing of dsDNA-Containing Bacteriophages Directly from a Single Plaque.- Preparing cDNA Libraries from Lytic Phage Infected Cells for Whole Transcriptome Analysis by RNA-Seq.- Essential Steps in Characterizing Bacteriophages: Biology, Taxonomy, and Genome Analysis.- Annotation of Bacteriophage Genome Sequences using DNA Master: An Overview.- Phage Genome Annotation using the RAST Pipeline.- Visualization of Phage Genomic Data: Comparative Genomics and Publication-Quality Diagrams.- Transposable Bacteriophages as Genetic Tools.- Applications of the Bacteriophage Mu In Vitro Transposition Reaction and Genome Manipulation via Electroporation of DNA Transposition Complexes.- Use of RP4::mini-Mu for Gene Transfer.- Muprints and Whole Genome Insertion Scans: Methods for Investigating Chromosome Accessibility and DNA Dynamics using Bacteriophage Mu.
Erscheinungsdatum | 08.12.2017 |
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Reihe/Serie | Methods in Molecular Biology ; 1681 |
Zusatzinfo | 36 Illustrations, color; 25 Illustrations, black and white; XI, 316 p. 61 illus., 36 illus. in color. With online files/update. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 177 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | bacterial virus research • CRISPR/cas genome editing • gene predictions • genome sequences • High-throughput sequencing • RNAseq • Synthetic biology • systematic transcriptome analysis • systematic transcriptome analysis using RNAseq |
ISBN-10 | 1-4939-7341-X / 149397341X |
ISBN-13 | 978-1-4939-7341-5 / 9781493973415 |
Zustand | Neuware |
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