Methods in Tau Cell Biology

Dr. Feinstein earned his undergraduate degree in Biochemistry at the University of California, Berkeley and his Ph.D. from the Department of Biochemistry and Biophysics at the University of California School of Medicine, San Francisco. Subsequently, he was a postdoctoral fellow in the Department of Neurobiology at the Stanford University School of Medicine until 1986, at which time he joined the faculty at UCSB. He has served as a grant reviewer for the National Institutes of Health, the National Science Foundation and the Cancer Research Coordinating Committee of California. In 2011, he was appointed a National Academies Education Fellow in the Life Sciences by the National Academies of Science and previously has been awarded a UCSB Distinguished Teaching Award. Dr. Feinstein is presently Co-Director of the Neuroscience Research Institute. Dr. Lapointe has earned her undergraduate degree in Biology & Psychology at the University of Minnesota-Morris, and her Ph.D. in Neuroscience from Northwestern Univeristy. Subsequently, she was a postdoctoral fellow in Univeristy of California, Santa Barbara until 2013, and has continued to serve UCSB as an associate specialist. She is now an assistant project scientist at UCSB, and has participated in a number of research projects and publications, which her research is mainly focused on Tau proteins.

1. Recombinant tau expression and purification
Stuart Feinstein
2. In vitro MT dynamics and MT ends
Nichole LaPointe
3. Methods related to investigating tau structure and MT bundling
Cyrus R. Safinya
4. Neurite outgrowth and retraction
Brett Cook
5. Methods related to studying tau fragmentation
Adrianna Ferrerira
6. Isoform-specific differences in tau in vitro aggregation
Truman C. Gamblin
7. Cosedimentation assays of microtubule binding
Holly Goodson
8. Methods to study tau aggregation
Songi Han
9. Methods to study tau phosphorylation
Diane Hanger
10. Structure of tau oligomers
Nicholas M. Kanaan
11. Use of mouse models for studies of pathological tau action
Virginia Lee and Sneha Narasimhan
12. Application of FRET and FCS to study tau structure-function
Elizabeth Rhoades
13. Use of the fly system to study tau action
Michelle Steinhilb
14. Single molecule assays with motor proteins
Christopher Berger
15. Tau clearance mechanisms in primary neurons with a focus on autophagy
Gail Johnson
16. Structural studies of tau
Eckhard Mandelkow
17. TIRF assays of tau effects on MT and actin cytoskeletons in vitro
Isabelle Arnal
18. TIRF assays of tau interactions with EB1
Isabelle Arnal
19. A novel approach to address tau biology, pathology, and propagation in vivo in mice using Adeno associated viruses
Susanne Wegmann