Dyneins

Stephen M. King is Professor of Molecular Biology and Biophysics at the University of Connecticut School of Medicine and is also director of the electron microscopy facility. He has studied the structure, function and regulation of dyneins for over 30 years using a broad array of methodologies including classical/molecular genetics, protein biochemistry, NMR structural biology and molecular modeling, combined with cell biological approaches, imaging and physiological measurements.

I. History and Evolution 1. Discovery of dynein and its properties: a personal account 2. Origins of cytoplasmic dynein 3. The evolutionary biology of dyneins

II. Dyneins in Ciliary Biology 4. Cytoplasmic preassembly and trafficking of axonemal dyneins 5. Composition and assembly of axonemal dyneins 6. Organization of dyneins in the axoneme 7. Genetic approaches to axonemal dynein function in Chlamydomonas and other organisms 8. Regulatory mechanics of outer arm dynein motors 9. Control of axonemal inner dynein arms 10. Ciliary and flagellar motility and the nexin-dynein regulatory complex 11. Regulation of dynein-driven ciliary and flagellar movement 12. Dynein-mediated photobehavioral responses in Chlamydomonas 13. Dynein and intraflagellar transport

III. Cytoplasmic Dynein Biology 14. Cytoplasmic dynein function defined by subunit composition 15. Regulation of cytoplasmic dynein motility 16. Insights into cytoplasmic dynein function and regulation from fungal genetics 17. Role of dynactin in dynein-mediated motility 18. Role of cytoplasmic dynein and dynactin in mitotic checkpoint silencing 19. Cytoplasmic dynein during mitosis 20. Dynein and dynactin at microtubule plus ends 21. Drosophila cytoplasmic dynein: mutations, tools and developmental functions