The Handbook of Histopathological Practices in Aquatic Environments

Dr Costa is a Marine Biologist specializing in Environmental Toxicology, with over 2 decades of experience in histology and histopathology, with emphasis on marine animals, both vertebrates and invertebrates. His expertise as a histologist ranges between mollusks and crustaceans to fish and known model organisms such as the zebrafish and murids. Whether using them biomarker candidates in environmental monitoring or as phenotypic anchors in complex and multidisciplinary toxicological studies, he has been applying and developing many histopathological techniques. He additionally teaches these techniques to graduate and post-graduate students with diverse academic backgrounds.

1. Introduction2. General concepts3. Sample preparation4. Staining protocols5. Microphotography6. Qualitative, semi-quantitative and quantitative histopathology7. Recipes8. Troubleshooting