A Structure-Function Toolbox for Membrane Transporter and Channels

Prof. Dr. Christine Ziegler works at the Department of Structural Biology, Max Planck Institute of Biophysics, Germany

1. Lipid Nanodiscs as a Tool for High-Resolution Structure Determination of Membrane Proteins by Single-Particle Cryo-EM 2. SSM-Based Electrophysiology for Transporter Research 3. Saposin-Lipoprotein Scaffolds for Structure Determination of Membrane Transporters 4. Single-Molecule Fluorescence Studies of Membrane Transporters Using Total Internal Reflection Microscopy 5. The MEMbrane Protein Single ShoT Amplification Recipe: MemStar 6. Efficient Screening and Optimization of Membrane Protein Production in Escherichia coli 7. Pinning Down the Mechanism of Transport: Probing the Structure and Function of Transporters Using Cysteine Cross-Linking and Site-Specific Labeling 8. Ion Channel Conformation and Oligomerization Assessment by Site-Directed Spin Labeling and Pulsed-EPR 9. Strategy for the Thermostabilization of an Agonist-Bound GPCR Coupled to a G Protein