The HPLC Expert II (eBook)

Stavros Kromidas studied biology and chemistry at the University of Saarbrücken, where he obtained his Ph.D. degree on the development of new chiral stationary phases for HPLC in 1983. After working for Waters GmbH for five years, he founded NOVIA GmbH, a provider of professional training and consulting in analytical chemistry, serving as the CEO until 2001. Since 2001 he works as an independent consultant for analytical chemistry, based in Saarbrücken (Germany). For more than 20 years he has regularly held lectures and training courses on HPLC, and has authored numerous articles and several books on various aspects of chromatography.

Introduction: When should I use my UHPLC as a UHPLC?

PART 1: HARDWARE AND SOFTWARE, SEPARATION MODES, MATERIALS
Modern HPLC/UHPLC equipment
Requirements for the different parts of HPLC/UHPLC equipment
The column oven - a simple matter?
The problem of the extra column dispersion in an HPLC/UHPLC equipment
The gradient: Requirements, optimal operation, tricks and pitfalls
Hardware requirements for LC coupling with different mass spectrometers
2D-Chromatography - potentials and limitations
Selecting the right column materials in HPLC and UHPLC
Qality by Design in HPLC Method Development

PART 2: USE CASES AND TRENDS
What should the software be able to do, so that the hardware can be used to its full potential?
Aspects of modern HPLC - a user´s experience report
Hints and recommendations for optimal use of HPLC equipment
Using UHPLC to solve the compound separation problem
Equipment review (Agilent, Shimadzu, ThermoScientific)