Telomeres and Telomerase
Humana Press Inc. (Verlag)
978-1-4939-6891-6 (ISBN)
Cutting-edge and thorough, Telomeres and Telomerase: Methods and Protocols, Third Edition is a valuable and informative resource for anyone who is interested in the developing field of telomeres and telomerase.
Introduction to Telomeres and Telomerase.- Analysis of Average Telomere Length in Human Telomeric Protein Knockout Cells Generated by CRISPR/Cas9.- Telomere Length Analysis by Quantitative Fluorescent In Situ Hybridization (Q-FISH).- Telomere Strand-Specific Length Analysis by Fluorescent In Situ Hybridization (Q-CO-FISH).- Telomere G-Rich Overhang Length Measurement: DSN Method.- Telomere G-Overhang Length Measurement Method 2: G-Tail Telomere HPA.- Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In.- Analysis of Yeast Telomerase by Primer Extension Assays.- Assessing Telomerase Activities in Mammalian Cells Using the Quantitative PCR-Based Telomeric Repeat Amplification Protocol (qTRAP).- Telomeres and NextGen CO-FISH: Directional Genomic Hybridization (Telo-dGH™).- Visualization of Human Telomerase Localization by Fluorescence Microscopy Techniques.- Cytogenetic Analysis of Telomere Dysfunction.- Probing the Telomere Damage Response.- Induction of Site-Specific Oxidative Damage at Telomeres by Killerred-Fused Shelretin Proteins.- Using Protein-Fragment Complementation Assays (PCA) and Peptide Arrays to Study Telomeric Protein–Protein Interactions.- In Vitro Preparation and Crystallization of Vertebrate Telomerase Subunits.- Human Telomeric G-Quadruplex Structures and G-Quadruplex-Interactive Compounds.- Analysis of Telomere-Homologous DNA with Different Conformations Using 2D Agarose Electrophoresis and In-Gel Hybridization.- Analysis of Telomere Proteins by ChromatinImmunoprecipitation (ChIP).
Erscheinungsdatum | 07.04.2017 |
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Reihe/Serie | Methods in Molecular Biology ; 1587 | Methods in Molecular Biology ; 1587 |
Zusatzinfo | 18 Illustrations, color; 19 Illustrations, black and white; XI, 218 p. 37 illus., 18 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Schlagworte | DSN Method • G-Tail Telomere HPA • Q-CO-FISH • Q-FISH • qTRA |
ISBN-10 | 1-4939-6891-2 / 1493968912 |
ISBN-13 | 978-1-4939-6891-6 / 9781493968916 |
Zustand | Neuware |
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