Protein Terminal Profiling
Humana Press Inc. (Verlag)
978-1-4939-6849-7 (ISBN)
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Cutting-edge and thorough, Protein Terminal Profiling: Methods and Protocols is a valuable resource for researchers that focus on biochemistry and cell biology, and those who share a broad interest in protein functionality and protein modifications.
[14C]-Acetyl-CoenzymeA-Based In Vitro N-Terminal Acetylation Assay.- DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity.- SILProNAQ: A Convenient Approach for Proteome-Wide Analysis of Protein N-Termini and N-Terminal Acetylation Quantitation.- Profiling of Protein N-Termini and their Modifications in Complex Samples.- Protease Substrate Profiling by N-Terminal COFRADIC.- Doublet N-Terminal Oriented Proteomics for N-Terminomics and Proteolytic Processing Identification.- Multidimensional Analysis of Protease Substrates and their Cellular Origins in Mixed Secretomes from Multiple Cell Types.- System-Wide Profiling of Protein Amino Termini from Formalin-Fixed, Paraffin-Embedded Tissue Specimens for the Identification of Novel Substrates.- Identification of Carboxypeptidase Substrates by C-Terminal COFRADIC.- ProC-TEL: Profiling of Protein C-Termini by Enzymatic Labeling.- Determining Protease Substrates within a Complex Protein Background using the PROtein TOpography and Migration Analysis Platform (PROTOMAP).- Multiplexed Protease Specificity Profiling Using Isobaric Labeling.- FPPS - Fast Profiling of Protease Specificity.- Profiling of Protease Cleavage Sites by Proteome-Derived Peptide Libraries and Quantitative Proteomics.- Prediction of Proteases Involved in Peptide Generation.- Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic Approaches.- Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors.- Simultaneous Detection of Metalloprotease Activities in Complex Biological Samples Using the PrAMA (Proteolytic Activity Matrix Assay) Method.- Synthesis and Application of Activity-Based Probes for Proteases.
Erscheinungsdatum | 06.04.2017 |
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Reihe/Serie | Methods in Molecular Biology ; 1574 |
Zusatzinfo | 37 Illustrations, color; 16 Illustrations, black and white; XII, 268 p. 53 illus., 37 illus. in color. With online files/update. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | ChaFRADIC • COFRADIC • N-CLAP • N-terminal acetylation • N-terminal profiling • protease specificity profiling • protein N- and C-termini • PTAG |
ISBN-10 | 1-4939-6849-1 / 1493968491 |
ISBN-13 | 978-1-4939-6849-7 / 9781493968497 |
Zustand | Neuware |
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