Kidney Research -

Kidney Research

Experimental Protocols
Buch | Softcover
276 Seiten
2016
Humana Press Inc. (Verlag)
978-1-4939-5640-1 (ISBN)
149,79 inkl. MwSt
As the expense of treating a growing number of end-stage kidney disease patients increases, greater attention has been paid to prevention and early treatment. The study of renal disease, however, suffers due to the complex nature of renal anatomy and physiology and the plethora of different cell types found in the kidney. In Kidney Research: Experimental Protocols, top experts in the field seek to aid researchers by providing a number of specialized techniques developed to examine this intricate system. Through both well-established and novel methods, this volume explores the preparation and culture of the main cell types used to study renal disease mechanisms, the common animal models used to mimic the various forms of human renal disease, and specific applications and techniques used in vivo and in vitro. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain step-by-step, readily reproducible laboratory protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.


Essential and cutting-edge, Kidney Research: Experimental Protocols delivers invaluable guidance to new and experienced laboratory researchers delving into the sophisticated study of the kidney.

Isolation and Propagation of Cell Populations.- Isolation and Propagation of Glomerular Mesangial Cells.- Isolation and Primary Culture of Human Proximal Tubule Cells.- Propagation and Culture of Renal Fibroblasts.- Animal Models.- Small Animal Models of Kidney Disease: A Review.- Techniques and Applications.- Measurement of Glomerular Filtration Rate in Conscious Mice.- Laser Capture Microdissection of Kidney Tissue.- Quantitative Gene Expression Analysis in Kidney Tissues.- In Vivo Imaging of Leukocyte Recruitment to Glomeruli in Mice Using Intravital Microscopy.- Using In Situ Hybridisation to Localize Renal Gene Expression in Tissue Sections.- Immuno and Lectin Histochemistry for Renal Light Microscopy.- Immuno and Lectin Histochemistry for Renal Electron Microscopy.- Pimonidazole Adduct Immunohistochemistry in the Rat Kidney: Detection of Tissue Hypoxia.- Identification of Apoptosis in Kidney Tissue Sections.- Cell-Populated Floating Collagen Lattices: An In Vitro Model of Parenchymal Contraction.- Mechanical Stretch-Induced Signal Transduction in Cultured Mesangial Cells.- Determination of Collagen Content, Concentration, and Sub-types in Kidney Tissue.- SELDI-TOF Mass Spectrometry-Based Protein Profiling of Kidney Tissue.- In Vivo Transfer of Small Interfering RNA or Small Hairpin RNA Targeting Glomeruli.

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology ; 466
Zusatzinfo 28 Illustrations, color; 24 Illustrations, black and white; XVI, 276 p. 52 illus., 28 illus. in color.
Verlagsort Totowa, NJ
Sprache englisch
Maße 155 x 235 mm
Themenwelt Medizinische Fachgebiete Innere Medizin Nephrologie
Medizin / Pharmazie Medizinische Fachgebiete Laboratoriumsmedizin
Medizin / Pharmazie Studium 1. Studienabschnitt (Vorklinik)
Studium 2. Studienabschnitt (Klinik) Pathologie
Naturwissenschaften Biologie Biochemie
Naturwissenschaften Biologie Genetik / Molekularbiologie
Naturwissenschaften Biologie Zellbiologie
ISBN-10 1-4939-5640-X / 149395640X
ISBN-13 978-1-4939-5640-1 / 9781493956401
Zustand Neuware
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