Metallomics (eBook)

Bernhard Michalke is leader of the research group "Elements and Element Speciation" and of the "Central Inorganic Analysis Division" as well as deputy director of the research unit "Analytical BioGeoChemistry" at the Helmholtz Center Munich - German Research Center for Environmental Health. Since 1996 he has been lecturer at the Institute of Analytical Chemistry and Food Chemistry of the Technical University of Graz, Austria, where he received his habilitation in analytical chemistry in 1999. Since 1988, he is principal researcher at the Helmholtz Center Munich. His reasearch interests encompass speciation projects related to environmental health, i.e. the exposure and chemical speciation of neuro-degenerative elements such as manganese and species related neuro-toxic effects in the central nervous system. He has published more than 170 peer reviewed articles and 10 book chapters. Since 1998, he has been co-organizer of several international conferences.

Preface

PART I. Analytical Methods and Strategies in Metallomics

THE POSITION OF METALLOMICS WITHIN OTHER OMICS FIELDS
Introduction
Metallome and Metallomics in Relation to Other "-Ome" and "-Omics" Fields
Is Metallomics Feasible as a Global Study of the Metallome
Approaching the Metallome: Study of Metallome Subgroups
Analytical Strategies in Metallomics
Functional Connections Between DNA, Proteins, Metabolites, and Metals
Metallothiolomics as Example for Metallomics Studies of a Metallome Subgroup
Concluding Remarks

COUPLING TECHNIQUES AND ORTHOGONAL COMBINATION OF MASS SPECTROMETRIC TECHNIQUES
Introduction
Analytical Techniques for Metallomics
Ionization Principles and Mass Spectrometric Detectors for Speciation
Overview about Coupling Techniques
Finals Remarks and Outlook

QUALITY CONTROL IN SPECIATION ANALYSIS USING HPLC WITH ICP-MS AND ESI MS/MS: FOCUS ON QUANTITATION STRATEGIES USING ISOTOPE DILUTION ANALYSIS
Introduction
Synergetic Use of Elemental and Organic Mass Spectrometry in Compound Quantitation and Quality Assurance of Food Selenium Speciation
The Role of Species-Specific Isotopic Dilution in Increasing Metrological Traceability for the Quantification of Bioinorganic Species

NOVEL METHODS FOR BIOIMAGING INCLUDING LA-ICP-MS, NanoSIMS, TEM/X-EDS, AND SXRF
Introduction
Bioimaging by LA-ICP-MS
Bioimaging by NanoSIMS
Bioimaging by TEM/X-EDS
Bioimaging by SXRF
Conclusions and Outlook

ELECTROCHEMISTRY COUPLED TO MASS SPECTROMETRY FOR INVESTIGATING OXIDATIVE METABOLISM OF PT-BASED DRUG CONJUGATES: A NOVEL APPROACH
Introduction
EC-MS Methodology
EC-MS of Thiols
Influence of Cisplatin on Thiol Oxidation
Conclusions

PART II. Metallomics in Environment and Nutrition

SELENIUM AND SELENIUM SPECIES
Speciation Analysis Especially of Tin and Selenium in Environmental Matrices
Selenium Species Extraction and Speciation in Plants and Yeast

ARSENIC AND AS SPECIES
Arsenic Species in Marine Food
Compounds with As?S Bonds: Analytical and Biogeochemical Reasons Why These Species have been Elusive in Biota and Environment
Arsenolipids: An Overview of Current Analytical Aspects

ANALYTICAL PROCEDURES FOR SPECIATION OF CHROMIUM, ALUMINUM, AND TIN IN ENVIRONMENTAL AND BIOLOGICAL SAMPLES
Speciation of Chromium
Speciation of Aluminum
Speciation of Tin

MERCURY TOXICITY AND SPECIATION ANALYSIS
Mercury Toxicity
Mercury Speciation Analysis

ENVIRONMENTAL SPECIATION OF PLATINUM EMISSIONS FROM CHEMOTHERAPY
Introduction
Elemental Analysis of Platinum
Quantification Strategies
Preparation of Samples for Total Platinum Analysis by ICP-MS
Analysis of Platinum
Speciation of Platinum Emissions from Chemotherapy
Speciation Strategies for the Determination of CPC
Selected Applications
Conclusion

NANOPARTICLES IN ENVIRONMENT AND HEALTH EFFECT
Introduction
Nanoparticle Overview
Analytical Strategies
Conclusion

PART III. Metallomics in Medicine and Biology

METALLOPROTEINS
General Introduction to Metalloprotein Analysis
Sample Preparation Methodologies to Preserve Metal-Protein Interactions
Analytical Strategies for Identification of Metalloproteins
Quantitative Strategies for the Analysis of Metalloproteins

BIOMEDICAL AND PHARMACEUTICAL APPLICATIONS
Selenium and Selenoproteins in Human Health and Diseases
Metal Species as Biomarkers for Medical Diagnosis: A Case Study of Alzheimer's Disease
Vanadium Speciation as a Means in Drug Development and Monitoring for Diabetes
Analysis of Pt- and Ru-Based Anticancer Drugs: New Developments
Silver Distribution in Skin during Wound Healing
Neurodegeneration with Focus on Manganese and Iron Speciation

Index