Fundamentals of MALDI-ToF-MS Analysis (eBook)

Dr. Samira Hosseini obtained her BSc degree in Applied Physics, her MSc degree in Polymer Chemistry and her PhD degree in Biomedical Engineering. Currently, she is working as a Postdoctoral Researcher at School of Engineering and Sciences, Tecnológico de  Monterrey, Mexico. Her main areas of  research are surface engineering, interface science, bioanalytical systems and polymeric materials.Sergio O. Martinez is a professor of Electrical and Computer Engineering at Tecnológico de Monterrey, Monterrey Campus; and Chair of the Sensors and Devices of the National School of Engineering and Sciences at the same Institute. He obtained his Ph.D. in Microelectronics from the National Polytechnic Institute of Grenoble, France in 2002. He  is a member of the Mexican Research National System since 2004 and the Mexican Academy of Sciences since 2010. His recent research activities, focused on Micro/Nanotechnolgies, include Microfabrication Processes for Particle Manipulation Devices, Electrokinetically-driven Microdevices for Manipulation of Suspended Particles in Fluidic Media, and Modeling and Design of SPR-based Biosensing Platforms. He has been Principal Investigator in projects funded by CONACYT, the Mexican Economy Ministry, and the Bureau of Science and Technology of Nuevo Leon. He has authored/coauthored 75 peer-reviewed publications, 7 book chapters and 2 granted patents.

Fundamentals of MALDI-ToF-MS analysisAbstract1. 1 Background of MALDI-ToF-MS1.2 Applications of MALDI-ToF-MS1.2.1 Biochemistry1.2.2 Organic chemistry1.2.3 Polymer chemistry1.2.4 Microbiology1.2.5 Medicine1.3 Ionization mechanism1.4 Time of flight mass spectrometry mechanism1.5 Detection modes1.5.1 Linear mode1.5.2 Reflection mode1.6 Laser range in MALDI1.7 Matrix selection1.7.1 Limitations of matrices1.8 Ionizing agent selection1.9 Sample preparation1.9.1 Analyzable and non-analyzable polymers1.9.2 Sample preparation techniques1.9.2.1. Dried-droplet1.9.2.2. Fast crystallization1.9.2.3. Spin coating1.9.2.4. Layer by layer deposition1.9.2.5. Electrospray deposition1.10 Mass discrimination1.10.1 Alternative solutions for overcoming mass discrimination1.11 MALDI coupling1.11.1 Ion-mobility time of flight mass spectrometry (IMS-TOF-MS)1.11.2 Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS)1.11.3 Tin layer chromatography (TLC)1.11.4 Size exclusion chromatograph (SEC)References2 Application of MALDI-ToF-MS analysis in bio-diagnostic domainAbstract2.1 Biosensors2.2 Synthesis of poly methyl methacrylate-co-methacrylic acid P(MMA-co-MAA) andpreparation of the biochips2.3 Sample preparation for MALDI analysis2.4 MALDI data interpretation2.4.1 Different species as the possible products of soft ionization2.4.2 General structure of P(MMA-co-MAA)2.4.3 MALDI analysis of PMMA2.4.4 MALDI analysis of P(MMA-co-MAA)2.4.5 End-group analysis2.5 Role of MALDI analysis in bio-sensing application of P(MMA-co-MAA)2.5.1 Reaction's parameters2.5.2 Chemistry aspect2.5.3 Physical aspectReferences3 Application of MALDI-ToF-MS analysis in tissue engineeringAbstract3.1 Tissue Engineering3.2 Synthesis of poly octanediol citrate acid-sebacate acid P(OCS) and preparation of thescaffold3.3 MALDI analysis of P(OCS) and sample preparation3.4 MALDI data interpretation3.5 Role of MALDI analysis in tissue engineering application of P(OCS)3.5.1 Reaction's parameters3.5.2 Chemistry aspect3.5.3 Mechanical aspectReferences4 Application of MALDI-ToF-MS analysis in drug deliveryAbstract4.1 Drug delivery4.2 Synthesis of PCLT-CA and preparation of elastomeric films and coatings4.3 Sample preparation for MALDI analysis4.4 MALDI data interpretation4.5 The role of MALDI analysis in drug delivery application of PCLT-CA4.5.1 Chemistry aspect4.5.2 Mechanical aspectReferencesSummary