Exosomes and Microvesicles

Andrew Hill, Associate Professor/Principle Research Fellow, Department of Biochemistry and Molecular Biology, The University of Melbourne Bio21 Institute,  Email: a.hill@unimelb.edu.au

Methods to Analyze EVs.- Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles.- Immuno-Characterization of Exosomes using Nanoparticle Tracking Analysis.- Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy.- Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR.- Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM™ System.- A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration.- Multiplexed Phenotyping of Extracellular Vesicles using Protein Microarray (EV Array).- Purification and Analysis of Exosomes Released by Mature Cortical Neurons following Synaptic Activation.- A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space.- Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission.- Isolation of Platelet-Derived Extracellular Vesicles.- Bioinformatics Tools for Extracellular Vesicles Research.- Preparation and Isolation of siRNA-Loaded Extracellular Vesicles.- Interaction of Extracellular Vesicles with Endothelial Cells under Physiological Flow Conditions.