Antimicrobial Peptides

Antimicrobial Peptides: An Introduction.- Tools for designing amphipathic helical antimicrobial peptides.- Chemical Synthesis of Antimicrobial Peptides.- Microwave-Assisted Synthesis of Antimicrobial Peptides.- High Performance Liquid Chromatography and Mass Spectrometry-Based Design of Protolytically Stable Antimicrobial Peptides.-  Determination of Structure and Micellar Interactions of Small Antimicrobial Peptides by Solution-State NMR.- Mass spectrometry approaches for determining the structure of antimicrobial peptides.- Molecular Dynamics Simulation and Analysis of the Antimicrobial Peptides-Lipid Bilayers Interactions.- Calorimetry methods to study membrane interactions and perturbations induced by antimicrobial host defense peptides.- Fluorescence and absorbance spectroscopy methods to study membrane perturbations by antimicrobial host defense peptides.-  Applying Fluorescence Correlation Spectroscopy to InvestigatePeptide-Induced Membrane Disruption.- Macromolecule Biosynthesis Assay and Fluorescence Spectroscopy Methods to Explore Antimicrobial Peptide Mode(s) of Action.- Using Confocal Microscopy and Computational Modeling to Investigate the Cell Penetrating Properties of Antimicrobial Peptides.- Atomic Force Microscopy Study of the Interactions of Indolicidin with Model Membranes and DNA.- Protocols for studying the interaction of MSI-78 with the Membranes of Whole Gram-positive and Gram-negative Bacteria by NMR.- Preparation of membrane models of Gram-negative bacteria and their interaction with Antimicrobial Peptides studied by CD and NMR.- Studying the Interaction of Magainin 2 and Cecropin A with E. coli Bacterial Cells using Circular Dichroism.- Studying the Mechanism of Membrane Permeabilization induced by Antimicrobial Peptides using Patch-clamp Techniques.- Assays for Identifying Inducers of the Antimicrobial Peptide LL-37.- Methods for Elucidating the Mechanism of Action of Proline-rich and other Non-lytic Antimicrobial Peptides.- The Interaction of Antimicrobial Peptides with the Membrane and Intracellular Targets of Staphylococcus Aureus Investigated by ATP-leakage, DNA-binding Analysis and the Expression of a LexA Controlled Gene, recA.- Methods for Investigating Biofilm Inhibition and Degradation by Antimicrobial Peptides.- Protocols for Studying Inhibition and Eradication of Bacterial Biofilms by Antimicrobial Peptides.- Protocols for Studying Antimicrobial Peptides (AMPs) as Anti-cancer Agents.- Using Oral and Colon Cancer Cells for studying the Anti-cancer properties of Antimicrobial peptides.- Using Disease-Associated Enzymes to Activate Antimicrobial Peptide Prodrugs.- Anti-inflammatory Properties of Antimicrobial Peptides and Peptidomimetics: LPS and LTA Neutralization.- Protocols for Screening Antimicrobial Peptides That Influence Virulence Gene Expression in Staphylococcus aureus.- Methods for in vitro Analysis of Antimicrobial Activity and Toxicity of Anti-keratitis Peptides: Bacterial Viability in tears, MTT and TNF-a release assays.- Methods for In Vivo/ex-vivo Analysis of Antimicrobial Peptides in Bacterial Keratitis: siRNA Knockdown, Colony Counts, Myeloperoxidase, Immunostaining, and RT-PCR Assays.- Haemolytic Activity of Antimicrobial Peptides.