The Inflammasome

Assessing b-amyloid-induced NLRP3 Inflammasome Activation in Primary Microglia.- Activating the NLRP3 Inflammasome using the Amyloidogenic Peptide IAPP.- Assessment and Quantification of Crystal-induced Lysosomal Damage.- Assessment of Inflammasome Activation in Primary Human Immune Cells.- NLRP3 Inflammasome Activation and Cytotoxicity Induced by particulate Adjuvants.- Measuring Inflammasome Activation in Response to Bacterial Infection.- Detection of Pyroptosis by Measuring Released Lactate Dehydrogenase Activity.- ASC Speck Formation as a Read-out for Inflammasome Activation.- Immunoblotting for Active Caspase-1.-  Inflammasome Activation and Inhibition in Primary Murine Bone Marrow-Derived Cells, and Assays for IL-1α, IL-1β and Caspase-1.- Reconstituting the NLRP1 Inflammasome in vitro.- Assessing ATP Binding and Hydrolysis by NLR Proteins.- Blue Native Polyacrylamide Gel Electrophoresis to Monitor Inflammasome Assembly and Composition.- Analysis of Microbiota Alterations in Inflammasome-deficient Mice.- Quantification of Adipose Tissue Leukocytosis in Obesity.- In vivo Evaluation of Neutrophil Recruitment in Response to Sterile Particulates.