Gene Expression Profiling -

Gene Expression Profiling

Methods and Protocols

Lorraine O'Driscoll (Herausgeber)

Buch | Softcover
211 Seiten
2016 | Softcover reprint of the original 2nd ed. 2011
Humana Press Inc. (Verlag)
978-1-4939-6173-3 (ISBN)
74,89 inkl. MwSt
In this book, experts present a broad range of available and developing techniques for the analysis of gene expression at the DNA, RNA, and protein levels. A collection of clearly described and illustrated chapters includes tips and implementation advice.
Understanding gene expression and how it changes under normal and pathological conditions is essential to our understanding of the fundamentals of cell biology through to the targeted treatment of disease.  In Gene Expression Profiling: Methods and Protocols, Second Edition, experts in their particular fields compile detailed protocols for a broad range of techniques, currently available and being further developed, for the analysis of gene expression at the DNA, RNA, and protein levels. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and easy-to-use, Gene Expression Profiling: Methods and Protocols, Second Edition presents a collection of clearly described and illustrated chapters, certain to be helpful to researchers in academia, in hospitals, and in industry who are interested in applying techniques, whether basic or advanced, for the analysis of gene expression.

Real-Time PCR and Multiplex Approaches.- Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs.- Microarray Analysis of mRNAs: Experimental Design and Data Analysis Fundamentals.- Software and Tools for Microarray Data Analysis.- Analysis of Gene Expression as Relevant to Cancer Cells and Circulating Tumor Cells.- Gene Expression Profiling in Formalin-Fixed, Paraffin-Embedded Tissues Using the Whole-Genome DASL Assay.- MicroRNA Expression Analysis: Techniques Suitable for Studies of Intercellular and Extracellular MicroRNAs.- Western Blotting Analysis as a Tool to Study Receptor Tyrosine Kinases.- 2D Gel Electrophoresis and Mass Spectrometry Identification and Analysis of Proteins.- Design, Construction, and Analysis of Cell Line Arrays and Tissue Microarrays for Gene Expression Analysis.- Immunohistochemical and Immunofluorescence Procedures for Protein Analysis.- Advanced Microscopy: Laser Scanning Confocal Microscopy.- Isolation of Exosomes for Subsequent mRNA, MicroRNA, and Protein Profiling.- Atomic Force Microscopy (AFM) and High-Content Analysis (HCA): Two Innovative Technologies for Dissecting the Relationship Between Epithelial-Mesenchymal Transition (EMT)-Related Morphological and Structural Alterations and Cell Mechanical Properties.

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology ; 784
Zusatzinfo 15 Illustrations, color; 22 Illustrations, black and white; X, 211 p. 37 illus., 15 illus. in color.
Verlagsort Totowa, NJ
Sprache englisch
Maße 178 x 254 mm
Themenwelt Medizin / Pharmazie Medizinische Fachgebiete
Studium 2. Studienabschnitt (Klinik) Humangenetik
Naturwissenschaften Biologie Genetik / Molekularbiologie
ISBN-10 1-4939-6173-X / 149396173X
ISBN-13 978-1-4939-6173-3 / 9781493961733
Zustand Neuware
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