Cytochrome P450 Protocols

Bioluminescent Assays for Cytochrome P450 Enzymes. In Vitro Drug-Drug Interaction Assay.- High-Throughput Mass Spectrometric Cytochrome P450 Inhibition Screening.- The Synthesis, Characterization and Application of 13C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites.- High-Throughput Fluorescence Assay for Cytochrome P450 Mechanism-Based Inactivators.- Identification of Endogenous Substrates of Orphan Cytochrome P450 Enzymes Through the Use of Untargeted Metabolomics Approaches.- Genetic and Mass Spectrometric Tools for Elucidating the Physiological Function(s) of Cytochrome P450 Enzymes from Mycobacterium Tuberculosis.- An Escherichia coli Expression-Based Approach for Porphyrin Substitution in Heme Proteins.- Expression in Escherichia coli of a Cytochrome P450 Enzyme with a Cobalt Protoporphyrin IX Prosthetic Group.- Nanodiscs in the Studies of Membrane-Bound Cytochrome P450 Enzymes.- Rapid LC-MS Drug Metabolite Profiling Using Bioreactor Particles.- Fluorescence-Based Screening of cytochrome P450 activities in Intact Cells.- Screening for Cytochrome P450 Reactivity with a Reporter Enzyme.- High-Throughput Fluorescence Assay of Cytochrome P450 3A4.- Targeted Protein Capture for Analysis of Electrophile-Protein Adducts.- DNA Shuffling of Cytochrome P450 Enzymes.- Measurement of P450 Difference Spectra Using Intact Cells.- DNA Shuffling of Cytochromes P450 for Indigoid Pigment Production.- P450 oxidoreductase – Genotyping, Expression, Purification of Recombinant Protein and Activity Assessments of Wild-Type and Mutant Protein.- LICRED: A Versatile Drop-In Vector for Rapid Generation of Redox-Self-Sufficient Cytochromes P450.- Update on Allele Nomenclature for Human Cytochromes P450 and the Human Cytochrome P450 Allele (CYP-allele) Nomenclature Database.- Simultaneous in vivo Phenotyping of CYP Enzymes.- Detection of Regulatory Polymorphisms: High-Throughput Capillary DNase-I Footprinting.- Isolation of MouseHepatocytes.- Highly Efficient SiRNA and Gene Transfer into Hepatocyte-like HepaRG Cells and Primary Human Hepatocytes: New Means for Drug Metabolism and Toxicity Studies.