siRNA Design

What Parameters to Consider and Which Software Tools to Use for Target Selection and Molecular Design of Small Interfering RNAs.- Methods for Selecting Effective siRNA Target Sequences Using a Variety of Statistical and Analytical Techniques.- Designing Functional siRNA with Reduced Off-Target Effects.- Design and Screening of siRNAs Against Highly Structured RNA Targets.- Engineering Small Interfering RNAs by Strategic Chemical Modification.- The Design, Selection and Evaluation of Highly Specific and Functional siRNA Incorporating Unlocked Nucleobase Analogs.- The Design, Preparation, and Evaluation of Asymmetric Small Interfering RNA for Specific Gene Silencing in Mammalian Cells.- Design of Nuclease-Resistant Fork-Like Small Interfering RNA (fsiRNA).- Designing Dual-Targeting siRNA Duplexes Having Two Active Strands that Combine siRNA and MicroRNA-Like Targeting.- Strategies for Designing and Validating Immunostimulatory siRNAs.- Designing Efficient and Specific Endoribonuclease Prepared siRNAs (esiRNAs).- Short Hairpin RNA-Mediated Gene Silencing.- Design of Lentivirally Expressed siRNAs.- Bi-Functional Short Hairpin RNA (bi-shRNA): Design and Pathway to Clinical Application.- Design and Chemical Modification of Synthetic Short shRNAs as Potent RNAi Triggers.- Production and Application of Long dsRNA in Mammalian Cells.- Design of RNAi Reagents for Invertebrate Model Organisms and Human Disease Vectors.- Construction of shRNA Expression Plasmids for Silkworm Cell Lines Using Single-Stranded DNA and Bst DNA Polymerase.- Designing Effective amiRNA and Multimeric amiRNA Against Plant Viruses.- Downregulation of Plant Genes with MiRNA Induced Gene Silencing.