Tau Protein

Conformational Dynamics of Intracellular Tau Protein Revealed by CD and SAXS.- Global Conformation of Tau Protein Mapped by Raman Spectroscopy.- Molecular Dynamics Simulation of Tau Peptides for the Investigation of Conformational Changes Induced by Specific Phosphorylation Patterns.- Tau Interaction with Tubulin and Microtubules: From Purified Proteins to Cells.- X-Ray Structural Study of Amyloid-Like Fibrils of Tau Peptides Bound to Small-Molecule Ligands.- Detection and Quantification Methods for Fibrillar Products of In Vitro Tau Aggregation Assays.- Fourier Transform Infrared (FTIR) Spectroscopy, Ultraviolet Resonance Raman (UVRR) Spectroscopy and Atomic Force Microscopy (AFM) for Study of the Kinetics of Formation and Structural Characterization of Tau Fibrils.- Assays for the Screening and Characterization of Tau Aggregation Inhibitors.- Tau Oligomers as Pathogenic Seeds: Preparation and Propagation In Vitro and In Vivo.- Mass Spectrometry Analysis of Lysine Post-Translational Modifications of Tau Protein from Alzheimer’s Disease Brain.- The Study of Posttranslational Modifications of Tau Protein by Nuclear Magnetic Resonance Spectroscopy: Phosphorylation of Tau Protein by ERK2 Recombinant Kinase and Rat Brain Extract, and Acetylation by Recombinant Creb-Binding Protein.- Tag-Free Semi-Synthesis of Tau Protein.- Production of O-GlcNAc Modified Recombinant Tau in E.coli and Detection of Ser400 O-GlcNAc Tau In Vivo.- Two-Dimensional Electrophoresis Protocols to Analyze the Microtubule-Associated Tau Proteins from Several Biological Sources.- A Simple Method to Avoid Non-Specific Signal When Using Monoclonal Anti-Tau Antibodies in Western Blotting of Mouse Brain Proteins.- Flow Cytometry Analysis and Quantitative Characterization of Tau in Synaptosomes from Alzheimer’s Disease Brains.- In Vivo Microdialysis of Brain Interstitial Fluid for the Determination of Extracellular Tau Levels.- Proximity Ligation Assay: A Tool to Study Endogenous Interactions between Tau and its Neuronal Partners.- Finding MAPT Mutations in Frontotemporal Dementia and Other Tauopathies.- Tracking Tau in Neurons: How to Grow, Fix and Stain Primary Neurons for the Investigation of Tau in All Developmental Stages.- Tracking Tau in Neurons: How to Transfect and Track Exogenous Tau into Primary Neurons.- Image-Based Analysis of Intracellular Tau Aggregation by Using Tau-BiFC Cell Model.- FRET and Flow Cytometry Assays to Measure Proteopathic Seeding Activity in Biological Samples.- In Vivo Imaging of Tau Aggregates in the Mouse Retina.- In Vivo Hyperthermic Stress Model: An Easy Tool to Study the Effects of Oxidative Stress on Neuronal Tau Functionality in Mouse Brain.- Identification of Tau Toxicity Modifiers in the Drosophila Eye.- Regulation of Neurotrophic Factors during Pathogenic Tau-Aggregation: A Detailed Protocol for Double-Labeling mRNA by In Situ Hybridization and Protein Epitopes by Immunohistochemistry.- Pin1 Knockout Mice: A Model for the Study of Tau Pathology in Alzheimer's Disease.