The Mitotic Exit Network

Regulation of Mitotic Exit in Saccharomyces cerevisiae.- Methods of Synchronization of Yeast Cells for the Analysis of Cell Cycle Progression.- Analysis of Cell Cycle Progression in Saccharomyces cerevisiae Using the Budding Index and Tubulin Staining.- Determination of Cell Cycle Stage and Mitotic Exit Through the Quantification of the Protein Levels of Known Mitotic Regulators.- Cdc14 Localization as a Marker for Mitotic Exit: In Vivo Quantitative Analysis of Cdc14 Release.- In Vitro Analysis of Tem1 GTPase Activity and Regulation by the Bfa1/Bub2 GAP.- Analysis of Protein-Protein Interactions Between MEN Components.- A New Methodology for the Quantification of In Vivo Cdc14 Phosphatase Activity.- Analysis of SUMOylation in the RENT Complex by Fusion to a SUMO-Specific Protease Domain.- A Substrate Trapping Method for Identification of Direct Cdc14 Phosphatase Targets.- Localizing MEN Components by Indirect Immunofluorescence Analysis of Budding Yeast.- Analysis of the Localization of MEN Components by Live Cell Imaging Microscopy.- Evaluation of the Dynamicity of Mitotic Exit Network and Spindle Position Checkpoint Components on Spindle Pole Bodies by Fluorescence Recovery After Photobleaching (FRAP).- Asymmetric Localization of Components and Regulators of the Mitotic Exit Network at Spindle Pole Bodies.- Evaluation of the Nucleolar Localization of the RENT Complex to Ribosomal DNA by Chromatin Immunoprecipitation Assays.- Analysis of the Functionality of the Mitotic Checkpoints.- Cdc14 and Chromosome Condensation: Evaluation of the Recruitment of Condensin to Genomic Regions.- Studying the Role of the Mitotic Exit Network in Cytokinesis.- Hippo Signaling in Mitosis: An Updated View in Light of the MEN Pathway.