Handbook of Phycological Methods

Contributors; Editors' preface; Introduction; Part I. Isolation of Organelles and Membranes: 1. Chloroplasts from Euglena gracilis C. A. Price; 2. Nuclei and chromatin from Euglena gracilis D. E. Buetow; 3. Mitochondria from Chlorella Neil G. Grant; 4. Microbodies from Chlorogonium and Spirogyra Helmut Stabenau; 5. Isolation and characterization of diatom membranes Cornelius W. Sullivan; Part II. Analysis of Chemical Constituents: 6. Chlorophylls and carotenoids Arne Jensen; 7. Algal biliproteins Harold W. Siegelman and J. Helen Kycia; 8. Nucleic acids Rose Ann Cattolico; 9. Protein determination by dye binding Gary Kochert; 10. Carbohydrate determination by the phenol-sulfaric acid method Gary Kochert; 11. Glycerolipids and fatty acids of algae J. P. Williams; 12. Carrageenans and agars J. S. Craigie and C. Leigh; 13. ß-1,3-Glucans in diatoms and brown seaweeds Sverre Myklestad; 14. Alginic Acid Bjørn Larsen; 15. Phenolic compounds in brown and red algae Mark A. Ragan and J. S. Craigie; 16. Brown seaweeds: analysis of ash, fiber, iodine and mannitol Bjørn Larsen; 17. Quantitation of the macromolecular components of microalgae Gary Kochert; 18. ATP, ADP, and AMP determinations in water samples and algal cultures David M. Karl and Osmund Holm-Hansen; Part III. Enzymes: 19. Ribulose-bis-phosphate carboxylase in Chlamydomonas S. S. Badour; 20. Nitrate reductase in marine phytoplankton Richard W. Eppley; 21. Threonine deaminase in marine microalgae N. J. Antia and R. S. Kripps; 22. Phosphoenolpyruvate carboxylse of blue-green algae Brian Colman; 23. Aldolases of multicellular marine algae Kazutosi Nisizawa; 24. The glucosyltranserases of red algae Jerome F. Frederick; 25. Anion-activated adenosine triphosphates Paul G. Falkowski; 26. Extracellular acid phosphatase of Ocheomonas sanica N. J. Patni and S. Aaronson; Part IV. Physiological and Biochemical Processes: 27. Determination of photosynthetic rates and 14C photoassimilatory products of brown seaweeds Bruno P. Kremer; 28. Polargraphic measurements of respiration following light-dark transitions Alan D. Jassby; 29. Hill reaction and photophosphorylation with chloroplast perparations from Chlamydomonas reinhardi Stephen Lien; 30. Respiratory assimilations of 14C-labelled substrates by a microalga Keith E. Cooksey; 31. Respiratory processses in mitochondria Neil G. Grant; 32. Determination of protein synthesis during synchronous growth Dennis J. O'Kane and Raymond F. Jones; 33. Nucleic acid synthesis Rose Ann Cattolico; 34. Nitrogen fixation Timothy H. Mague; 35. Uptake of organic substrates Johan A. Hellebust and Yi-Hung Lin; 36. Release of organic substances C. Nalewajko; Part V. Nutrients: 37. Nitrate uptake Richard W. Eppley; 38. Phosphate uptake F. P. Healey; 39. Sulfate uptake Monica Lik-Shing Tsang, Robert C. Hodson and Jerome A. Schiff; 40. Uptake of silicic acid Farooq Azam and Sallie W. Chisholm; 41. Uptake of micronutrients and toxic metals Anthony G. Davies; Part VI. Ion Content and Transport: 42. Measurement of ionic content and fluxes in microalgae J. Barber; 43. Ion transport and ion-stimulated adenosine triphosphatases Cornelius W. Sullivan; Part VII. Inhibitors: 44. Inhibitors used in studies of algal metabolism S. S. Badour; Part VIII. Appendix; Part IX. Indexes.