Protein Chromatography
Humana Press Inc. (Verlag)
978-1-4939-6410-9 (ISBN)
Cutting-edge and thorough, Protein Chromatography: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the field of protein chromatography.
A Synopsis of Protein and their Purification.- Gel-Filtration Chromatography.- Immunoaffinity Chromatography: Concepts and Applications.- Avoiding Proteolysis During Protein Purification.- Scale-Up of Protein Purification: Downstream Processing Issues.- Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune Sources.- Protein Stability: Enhancement and Measurement.- Tagging Recombinant Proteins to Enhance Solubility and Aid Purification.- Storage and Lyophilisation of Pure Proteins.- Differential Precipitation and Solubilization of Proteins.- Ion-Exchange Chromatography: Basic Principles and Application.- Protein Quantitation and Analysis of Purity.- Purification of Proteins Fused to Maltose-Binding Protein.- Purification of Poly-Histidine-Tagged Proteins.- Purification of Antibodies Using Affinity Chromatography.- Optimized Generationof High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens.- Measuring Protein-Protein Interactions Using Biacore.- Hydrophobic Interaction Chromatography.
Erscheinungsdatum | 01.11.2016 |
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Reihe/Serie | Methods in Molecular Biology ; 1485 |
Zusatzinfo | 21 Illustrations, color; 19 Illustrations, black and white; X, 426 p. 40 illus., 21 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Biologie ► Biochemie | |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | avoiding proteolysis during chromatography • high-throughput systems • immunoaffinity chromatography • immuno-qPCR • Ion-exchange • physicochemical properties of the target protein • protein quantitation • protein stability and storage • purification of recombinant proteins • recombinant antibody production |
ISBN-10 | 1-4939-6410-0 / 1493964100 |
ISBN-13 | 978-1-4939-6410-9 / 9781493964109 |
Zustand | Neuware |
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