Skeletal Muscle Regeneration in the Mouse

Michael Kyba, Ph.D. Associate Professor and Lillehei Endowed Scholar, Lillehei Heart Institute NHH4.126, University of Minnesota, SE Minneapolis, MN 55455, USA

Eccentric
Contraction-Induced Muscle Injury: Reproducible, Quantitative, Physiological Models
to Impair Skeletal Muscle’s Capacity to Generate Force.- Volumetric
Muscle Loss.- Freeze Injury of the Tibialis Anterior
Muscle.- Synergist Ablation
as a Rodent Model to Study Satellite Cell Dynamics in Adult Skeletal Muscle.- Inducing
and Evaluating Skeletal Muscle Injury by Notexin and Barium Chloride.- Cardiotoxin Induced Injury and
Skeletal Muscle Regeneration.- Fibrosis-Inducing Strategies in Regenerating Dystrophic and Normal Skeletal
Muscle.- Isolation, Cryosection, and Immunostaining
of Skeletal Muscle.- Isolation of Mouse Periocular Tissue
for Histological and Immunostaining Analyses of the Extraocular Muscles and
their Satellite Cells.- Skeletal Muscle Tissue Clearing
for LacZ and Fluorescent Reporters, and Immunofluorescence Staining.- Isolation, Culture, Functional
Assays and Immunofluorescence of Myofiber-Associated Satellite Cells.- Transplantation-Based Quantitative
Assays for Satellite Cell Self-Renewal and Differentiation.- Non-Invasive Tracking of Quiescent
and Activated MuSC Engraftment Dynamics In Vivo.- Myogenic Progenitors from Mouse Pluripotent
Stem Cells for Muscle Regeneration.- Assaying Human Myogenic Progenitor
Cell Activity by Reconstitution of Muscle Fibers and Satellite Cells in Immunodeficient
Mice.- Methods for Mitochondria and Mitophagy
Flux Analyses in Stem Cells of Resting and Regenerating Skeletal Muscle.- Identification, Isolation, and
Characterization of Mesenchymal Progenitors in Mouse and Human Skeletal Muscle.- FACS Fractionation and Differentiation of Skeletal-Muscle
Resident Multipotent Tie2+ Progenitors.- In Vitro Assays to Determine
Skeletal Muscle Physiologic Function.- In Vivo Assessment of Muscle
Contractility in Animal Studies.- Functional Measurement of
Respiratory Muscle Motor Behaviors Using Transdiaphragmatic Pressure.- Assessment of the Contractile Properties
of Permeabilized Skeletal Muscle Fibers.- Analysis of Aerobic Respiration
in Intact Skeletal Muscle Tissue by Microplate-Based Respirometry.