Protein Tyrosine Phosphatases

The Extended Family of Protein Tyrosine Phosphatases.- Global RT-PCR and RT-qPCR Analysis of the mRNA Expression of the Human PTPome.- Expression, Purification, and Kinetic Analysis of PTP Domains.- Peptide Microarrays for Real Time Kinetic Profiling of Tyrosine Phosphatase Activity of Recombinant Phosphatases and Phosphatases in Lysates of Cells or Tissue Samples.- Tailor-Made Protein Tyrosine Phosphatases: In Vitro Site-Directed Mutagenesis of PTEN and PTPRZ-B.- Assays to Measure PTEN Lipid Phosphatase Activity In Vitro from Purified Enzyme or Immunoprecipitates.- Assessing the Biological Activity of the Glucan Phosphatase Laforin.- Discovery and Evaluation of PRL Trimer Disruptors for Novel Anticancer Agents.- Analyzing Pseudophosphatase Function.- Crystallization of PTP Domains.- NMR Spectroscopy to Study MAP Kinase Binding to MAP Kinase Phosphatases.- Visualizing and Quantitating the Spatiotemporal Regulation of Ras/ERK Signaling by Dual-Specificity Mitogen-Activated Protein Phosphatases (MKPs).- In Situ Proximity Ligation Assay (In Situ PLA) to Assess PTP-Protein Interactions.- Use of Dominant Negative/Substrate Trapping PTP Mutations to Search for PTP Interactors/Substrates.- Detection and Identification of Ligands for Mammalian RPTP Extracellular Domains.- Production of Osteoclasts for Studying Protein Tyrosine Phosphatase Signaling.- Functional Analysis of Protein Tyrosine Phosphatases in Thrombosis and Haemostasis.- Functional Analysis of Dual-Specificity Protein Phosphatases in Angiogenesis.- Studying Protein-Tyrosine Phosphatases in Zebrafish.- Live staining of Drosophila Embryos with RPTP Fusion Proteins to Detect and Characterize Expression of Cell-Surface RPTP Ligands.- Methods to Study Protein Tyrosine Phosphatases Acting on Yeast MAPKs.