Gap Junction Protocols

Analysis of Liver Connexin Expression
Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction.- DNA Methylation Analysis of Human
Tissue-Specific Connexin Genes.- Detection of Connexins in Liver Cells Using
Sodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis.- Immunohisto- and
Cytochemistry Analysis of Connexins.- Small Interfering RNA-Mediated
Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells.- Generation and Use of Trophoblast
Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy
and Labor.- Identification of Connexin43
Phosphorylation and S-Nitrosylation
in Cultured Primary Vascular Cells.- Preparation of Gap Junctions in
Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome
Analysis.- Scrape Loading/Dye Transfer Assay.- Microinjection Technique for Assessment of Gap
Junction Function.- Electroporation Loading and Dye
Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling.- Using Fluorescence Recovery After Photobleaching to Study Gap Junctional
Communication In Vitro.- Tracking Dynamic Gap Junctional Coupling in Live Cells by Local
Photo-Activation and Fluorescence Imaging.- A Functional Assay to Assess Connexin43-Mediated Cell-to-Cell Communication of
Second Messengers in Cultured Bone Cells.- Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method
for Studying Gap Junctions and Hemichannels.- Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for
the Measurement of Gap Junction Conductance.