Electrophysiology

Dr. phil.nat. Jürgen RETTINGER Product Manager Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen, GermanyJürgen Rettinger received his doctor degree in physics at Goethe-University in Frankfurt. in 1995. His focus as group leader at Max-Planck-Institute of Biophysics in Frankfurt was research of structure-function relationships of ligand-gated ion channels and teaching electrophysiology at Goethe-University Frankfurt, Shanghai Institutes of Biological Sciences and Fudan-University Shanghai. Since 2008 Jürgen Rettinger is working with Multi Channel Systems responsible as product manager for electrophysiology. Silvia SCHWARZ Research Scientist Shanghai Key Laboratory for Acupuncture Mechanism and Acupoint Function Fudan University Guoshoujing Rd. 199 Shanghai 201203, ChinaSilvia Schwarz finished her university education in biology and chemistry at Albert-Ludwigs-University Freiburg in 1974. Thereafter, she was educated and worked as teacher in biology and chemistry until 2008. Since then she works as research associate at Shanghai Research Center for Acupuncture and Meridians (Shanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function, Fudan Univ.). Silvia Schwarz’s main research focus is on antiviral effects of Chinese herbal drugs applying electrophysiological methods.Prof. Dr. rer.nat. Wolfgang SCHWARZ Professor for PhysicsInstitut für Biophysik, Goethe-Universität FrankfurtMax-von-Laue-Str. 1, 60438 Frankfurt a.M., GermanyShanghai Key Laboratory for Acupuncture Mechanism and Acupoint FunctionFudan UniversityGuoshoujing Rd. 199Shanghai 201203, ChinaWolfgang Schwarz received his doctor degree in physics at University of Saarland in 1975. Thereafter, he spent two years as postdoc at University of Washington Seattle working on characteristics of ion channels. In 1981 he habilitated in physiology at University of Saarland and received a position as head of a Cell Physiology Laboratory at Max-Planck-Institute of Biophysics Frankfurt. Wolfgang Schwarz’s main focus turned to structure-function and regulation of carrier transporters and teaching electrophysiology as professor for physics at Goethe-University Frankfurt, Shanghai Institutes of Biological Sciences and Fudan-University Shanghai.  Since 2004 Wolfgang Schwarz is head of the Cell Electrophysiology Laboratory of Shanghai Research Center for Acupuncture and Meridian (Shanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function, Fudan Univ.) and concurrent professor at Fudan University.

Chapter 11     Introduction1.1   INTRODUCTORY OVERVIEW1.2   HISTORY OF ELECTROPHYSIOLOGYChapter 22     Basics: Theory2.1   ELECTRICAL CHARACTERISTICS OF BIOLOGICAL MEMBRANES2.2   ION DISTRIBUTION AT BIOLOGICAL MEMBRANES2.3   DONNAN DISTRIBUTION AND NERNST EQUATION2.4   GOLDMAN-HODGKIN-KATZ EQUATIONChapter 33.    Basics: Methods3.1   RECORDING ELECTRICAL SIGNALS FROM BODY SURFACE3.2   THE EXAMPLE (ECG)3.3   RECORDING ELECTRICAL SIGNALS FROM TISSUE3.4   RECORDING ELECTRICAL SIGNALS FROM SINGLE CELLS3.5   APPLICATION OF THE VOLTAGE-CLAMP TECHNIQUE3.6   THE PATCH-CLAMP TECHNIQUEChapter 44     Automated Electrophysiology4.1   AUTOMATED TWO-ELECTRODE VOLTAGE CLAMP4.2   AUTOMATED PATCH CLAMPChapter 55     Ion-selective Channels5.1   GENERAL CHARACTERISTICS OF ION CHANNELS5.2   SPECIFIC ION CHANNELSChapter 66     Theory of Excitability6.1   THE HODGKIN-HUXLEY DESCRIPTION OF EXCITATION6.2   CONTINUOUS AND SALTATORY CONDUCTION OF ACTION POTENTIALS6.3   GENERATION AND TRANSMISSION OF ACTION POTENTIALS6.4   SUMMARY OF THE DIFFERENT TYPES OF POTENTIALS6.5   ACTION POTENTIAL IN NON-NERVE CELLSChapter 77     Carrier-mediated Transport7.1   GENERAL CHARACTERISTICS OF CARRIERS7.2   CARRIERS ARE LIKE CHANNELS WITH ALTERNATING GATESChapter 88     Examples of Application of Electrophysiology8.1   STRUCTURE-FUNCTION RELATIONSHIPS OF CARRIER PROTEINS8.2   STRUCTURE-FUNCTION RELATIONSHIPS OF ION CHANNELS8.3   VIRAL ION CHANNELSAppendixA1.   THE GRAPH THEORYA2.   INFLUENCE OF EXTERNAL ELECTRICAL AND MAGNETIC FIELDS A2.1. MAGNETOSTATIC FIELDSA3.   A LABORATORY COURSE:Important Physical UnitsList of Symbols and AbbreviationsIndex