Microfluidic Methods for Molecular Biology

Chang Lu is a Professor of Chemical Engineering at Virginia Tech. His research focuses on developing microfluidic biotechnologies for cellular and molecular analysis with recent emphasis on low-input epigenomic studies. Scott Verbridge is an Assistant Professor in the Virginia Tech Department of Biomedical Engineering and Mechanics, and the Virginia Tech - Wake Forest School of Biomedical Engineering and Sciences. His research is focused on developing engineered tumor models to better understand cell-microenvironment interactions, and their impact on tumor progression and therapy response.  

Microfluidic Platforms for Quantitative Biology Studies in Model Organisms.- Microfluidic Methods in Single Cell Biology.- Convective PCR Thermocycling with Smartphone-based Detection: A Versatile Platform for Rapid, Inexpensive, and Robust Mobile Diagnostics.- Forensic Typing of Single Cells using Droplet Microfluidics.- Microfluidic Approaches to Fluorescence In Situ Hybridization (FISH) for Detecting RNA targets in Single Cells.- Microfluidic Multistage Integration for Analysis of Circulating Exosomes.- Microfluidic Single-Cell Functional Proteomics.- Microfluidics for DNA and Protein Analysis with Multiplex Microbead-based Assays.- Single Cell Phenotypic Screening in Inverse Metabolic Engineering.- Microfluidic Paper-based Multiplexing Biosensors for Electrochemical Detection of Metabolic Biomarkers.- Droplet Microfluidics for Screening of Surface-Marker and Secretory Protein Expression.- Wire-guided Droplet Manipulation for Molecular Biology.- Mechanical and Electrical Principles for Separation of Rare Cells.- Microfluidics for High Throughput Cellular Isolation and Analysis in Biomedicine.- Microfluidics for Cell Culture.- Microfluidic Chromatin Immunoprecipitation for Analysis of Epigenomic Regulations.