Chemotaxis
Humana Press Inc. (Verlag)
978-1-4939-3478-2 (ISBN)
This second edition expands on the first edition with new chapters describing methods for studying
cell movement, molecular components involved in chemotaxis, spatiotemporal
dynamics of signaling components, and quantitative modeling, as well as several
updated chapters from the first edition. Various methods to investigate
directional cell growth and movements are presented in Chapters 1-20. These
chapters contains experimental procedures to visualize and measure migration
behaviors of different kinds of organisms, including chemotropism in the
budding yeast; cell growth and migration of D. discoideum; border cell
migration in Drosophila; chemotaxis of mouse and human neutrophils; and
HIV-induced T cell chemotactic response. Chemotaxis: Methods and Protocols,
Second Edition also contains microscopy procedures for studying breast cancer
cell migration, tumor cell invasion in vivo, and axon guidance. The book concludes
with Chapters 21-27 describing methods that measure spatiotemporal dynamics of
signaling components involved in chemotaxis; introduce imaging techniques, such
as TRIF, BRET, FRET, and single-molecule microscopy; and mathematical models of
experimentally generated chemoattractant gradients. Written in the highly
successful Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls.
Cutting edge and thorough,
Chemotaxis: Methods and Protocols, Second Edition is a valuable resource for
anyone who is interested in the diverse methodologies that are propelling
chemotaxis research forward.
In Situ Assays of Chemotropism during
Yeast Mating.- Imaging
Polarization in Budding Yeast Migration of Dictyostelium discoideum to the
Chemoattractant Folic Acid.- Mitochondrial
Stress Tests Using Seahorse Respirometry on Intact Dictyostelium discoideum Cells.- Studying
Chemoattractant Signal Transduction Dynamics in Dictyostelium by BRET.- Wave Patterns
in Cell Membrane and Actin Cortex Uncoupled from Chemotactic Signals.- Chemotactic
Blebbing in Dictyostelium Cells.- Dissecting
Spatial and Temporal Sensing in Dictyostelium
Chemotaxis Using a Wave Gradient Generator.- Employing Dictyostelium as an Advantageous 3Rs
Model for Pharmacogenetic Research.- Identification
of Associated Proteins by Immunoprecipitation and Mass Spectrometry Analysis.- Biochemical
Responses to Chemically Distinct Chemoattractants during the Growth and
Development of Dictyostelium.- Live Imaging of
Border Cell Migrationin Drosophila.- ShRNA-Induced
Gene Knock-Down In Vivo to Investigate Neutrophil Function.- Studying
Neutrophil Migration In Vivo Using Adoptive Cell Transfer.- Intravital
Two-Photon Imaging of Lymphocytes Crossing High Endothelial Venules and
Cortical Lymphatics in the Inguinal Lymph Node.- Flow
Cytometry-Based Quantification of HIV-Induced T Cell Chemotactic Response.- Visualizing
Cancer Cell Chemotaxis and Invasion in 2D and 3D.- 4D Tumorigenesis
Model for Quantitating Coalescence, Directed Cell Motility and Chemotaxis,
Identifying Unique Cell Behaviors and Testing Anti-Cancer Drugs.- An Experimental
Model for Simultaneous Study of Migration of Cell Fragments, Single Cells and
Cell Sheets.- Axon Guidance
Studies Using a Microfluidics-Based Chemotropic Gradient Generator.- Visualization of
Actin Assembly and Filament Turnover by In Vitro Multi-Color TIRF Microscopy.- Quantitative
Monitoring Spatiotemporal Activation of Ras and PKD1 Using Confocal Fluorescent
Microscopy.- Fluorescence
Readout of a Patch Clamped Membrane by Laser Scanning Microscopy.- Use of Resonance
Energy Transfer Techniques for In Vivo Detection of Chemokine Receptor
Oligomerization.- Multi-State
Transition Kinetics of Intracellular Signaling Molecules by Single-Molecule
Imaging Analysis.- Mathematics of
Experimentally Generated Chemoattractant Gradients.- Modeling
Excitable Dynamics of Chemotactic Networks.
| Erscheinungsdatum | 09.03.2016 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1407 |
| Zusatzinfo | 83 Illustrations, color; 35 Illustrations, black and white; XIV, 425 p. 118 illus., 83 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| Schlagworte | Axon Guidance • breast cancer cell migration • cell movement • directional cell growth • quantitative modeling • single-molecule microscopy • spatiotemporal dynamics of signaling components • tumor call invasion in vivo |
| ISBN-10 | 1-4939-3478-3 / 1493934783 |
| ISBN-13 | 978-1-4939-3478-2 / 9781493934782 |
| Zustand | Neuware |
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