Biochemistry

Reginald H. Garrett was educated in the Baltimore city public schools and at the Johns Hopkins University, where he received his Ph.D. in biology in 1968. Since that time, he has conducted research and taught biochemistry courses at the University of Virginia, where he is currently Professor of Biology. He is the author of numerous papers and review articles on biochemical, genetic, and molecular biological aspects of inorganic nitrogen metabolism. His early research focused on the pathway of nitrate assimilation in filamentous fungi. His investigations contributed substantially to our understanding of the enzymology, genetics, and regulation of this major pathway of biological nitrogen acquisition. More recently, he has collaborated in systems approaches to the metabolic basis of nutrition-related diseases. His research has been supported by grants from the National Institutes of Health, the National Science Foundation, and private industry. A member of the American Society for Biochemistry and Molecular Biology, Garrett is a former Fulbright Scholar, was twice Visiting Scholar at the University of Cambridge, and was Invited Professor at the University of Toulouse, France. Charles M. Grisham received his B.S. in chemistry from the Illinois Institute of Technology in 1969 and his Ph.D. in chemistry from the University of Minnesota in 1973. Following a postdoctoral appointment at the Institute for Cancer Research in Philadelphia, he became Professor of Chemistry at the University of Virginia, where he teaches biochemistry, introductory chemistry, and physical chemistry. He has authored numerous papers and review articles on active transport of sodium, potassium, and calcium in mammalian systems, on protein kinase C, and on the applications of NMR and EPR spectroscopy to the study of biological systems. His work has been supported by the National Institutes of Health, the National Science Foundation, the Muscular Dystrophy Association of America, the Research Corporation, the American Heart Association and the American Chemical Society. A member of the American Society for Biochemistry and Molecular Biology, Grisham held the Knapp Chair in Chemistry in 1999 at the University of San Diego; was Visiting Scientist at the Aarhus University Institute of Physiology, Aarhus, Denmark, for two years; and received a Research Career Development Award from the National Institutes of Health.

Part I: Molecular Components of Cells
1. The Facts of Life: Chemistry Is the Logic of Biological Phenomena
2. Water: The Medium of Life
3. Thermodynamics of Biological Systems
4. Amino Acids and the Peptide Bond
5. Proteins: Their Primary Structure and Biological Functions
6. Proteins: Secondary, Tertiary, and Quaternary Structure
7. Carbohydrates and the Glycoconjugates of Cell Surfaces
8. Lipids
9. Membranes and Membrane Transport
10. Nucleotides and Nucleic Acids
11. Structure of Nucleic Acids
12. Recombinant DNA, Cloning, Chimeric Genes, and Synthetic Biology
Part II: Protein Dynamics
13. Enzymes—Kinetics and Specificity
14. Mechanisms of Enzyme Action
15. Enzyme Regulation
16. Molecular Motors
Part III: Metabolism and Its Regulation
17. Metabolism: An Overview
18. Glycolysis
19. The Tricarboxylic Acid Cycle
20. Electron Transport and Oxidative Phosphorylation
21. Photosynthesis
22. Gluconeogenesis, Glycogen Metabolism, and the Pentose Phosphate Pathway
23. Fatty Acid Catabolism
24. Lipid Biosynthesis
25. Nitrogen Acquisition and Amino Acid Metabolism
26. Synthesis and Degradation of Nucleotides
27. Metabolic Integration and Organ Specialization
Part IV: Information Transfer
28. DNA Metabolism: Replication, Recombination, and Repair
29. T ranscription and the Regulation of Gene Expression
30. Protein Synthesis
31. Completing the Protein Life Cycle: Folding, Processing, and Degradation
32. The Reception and Transmission of Extracellular Information
Abbreviated Answers to Problems
Index