Steroid Receptor Methods

Bioinformatics.- Bioinformatics of Nuclear Receptors.- Phylogenetic Inference and Parsimony Analysis.- Purification Protocols.- Expression and Purification of Recombinant Human Progesterone Receptor in Baculovirus and Bacterial Systems.- High-Yield Purification of Functionally Active Glucocorticoid Receptor.- Production and Purification of Histidine-Tagged Dihydrotestosterone-Bound Full-Length Human Androgen Receptor.- Large Scale Production of Nuclear Receptor Ligand-Binding Domains.- Physical Structure of Nuclear Receptor-DNA Complexes.- Isolation of Steroid-Regulated Genes from the Uterus by mRNA Differential Display.- Identification of Nuclear Hormone Receptor Homologs by Screening Libraries with Highly Degenerate Oligonucleotide Probes.- Steroid Hormone-Binding Assays.- Use of [99mTc]Technetium-Labeled Steroids as Probes for Steroid Hormone Receptors.- Steroid Hormone Metabolites and Hormone Binding Assays.- Vitamin D3 Analog Screening.- Protein Interaction Assays.- Application of Green Fluorescent Protein to the Study of Dynamic Protein-Protein Interactions and Subcellular Trafficking of Steroid Receptors.- Knockout Mice and Steroid Receptor Research.- Yeast Two-Hybrid Screening for Proteins that Interact with Nuclear Hormone Receptors.- Isolation of a p300/CBP Cointegrator-Associated Protein Coactivator Complex.- Nonradioactive Photoaffinity Labeling of Steroid Receptors Using Western Blot Detection System.- Analysis of Steroid Hormone-Induced Histone Acetylation by Chromatin Immunoprecipitation Assay.- Analyzing the Contributions of Chromatin Structure in Nuclear Hormone Receptor Activated Transcription In Vivo.- Cotransfection Assays and Steroid Receptor Biology.- Estrogen Receptor mRNA In Situ Hybridization Using Microprobe System.- Cancer Research and Drug Discovery.- Solid Tumor Cancer Markers and Applications to Steroid Hormone Research.- Assessing Modulation of Estrogenic Activity of Environmental and Pharmaceutical Compounds Using MCF-7 Focus Assay.- Combinatorial Chemistry in Steroid Receptor Drug Discovery.- Identification of Nuclear Receptor Interacting Proteins Using Yeast Two- Hybrid Technology.