Isotope Labeling of Biomolecules – Labeling Methods

Zvi Kelman is the Director of the Biomolecular Labeling Laboratory (BL2), National Institute of Standards and Technology (NIST). He is also an Adjunct Professor in the Department of Cell Biology and Molecular Genetics at the University of Maryland, College Park and affiliated with the Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine. Zvi earned a B.Sc. in Agriculture from the Hebrew University in Jerusalem and a M.Sc. in Cell Biology from the Weizmann Institute of Science. After receiving a Ph.D. in Molecular Biology from Cornell University, he was a Helen Hay Whitney Foundation Post-Doctoral Fellow at Johns Hopkins University and Memorial Sloan-Kettering Cancer Center. Upon completion of his postgraduate training, he became a Life Technologies Professor at the Center for Advanced Research in Biotechnology (CARB), University of Maryland Biotechnology Institute (UMBI). Zvi moved to the University of Maryland, College Park in 2010 as a Professor in the Department of Cell Biology and Molecular Genetics. In 2011 he was recruited to NIST to direct the Biomolecular Labeling Laboratory.

Labeling in Prokarya1. Robust High Yield Methodologies for 2H and 2H/15N/13C Labelling of Proteins for Structural Investigations using Neutron Scattering and NMR Anthony P. Duff, Karyn L. Wilde, Agata Rekas, Vanessa Lake and Peter J. Holden2. Protein labeling in Escherichia coli with 2H, 13C and 15NJ. Todd Hoopes, Margaret A. Elberson, Renae J. Preston, Prasad T. Reddy and Zvi Kelman3. Escherichia Coli Auxotroph Host Strains for Amino Acid-Selective Isotope Labeling of Recombinant Proteins Myat T. Lin, Risako Fukazawa, Yoshiharu Miyajima-Nakano, Shinichi Matsushita, Sylvia K. Choi, Toshio Iwasaki and Robert B. Gennis4. 19F-Modified Proteins and 19F-Containing Ligands as Tools in Solution NMR Studies of Protein InteractionsNaima G. Sharaf and Angela M. Gronenborn5. Biopolymer Deuteration For Neutron Scattering and Other Isotope-Sensitive TechniquesRobert A. Russell, Christopher J. Garvey, Tamim A. Darwish, L. John R. Foster and Peter J. Holden6. Production of Bacterial Cellulose with Controlled Deuterium-Hydrogen Substitution for Neutron Scattering StudiesHugh O’Neill, Riddhi Shah, Barbara Evans, Junhong He, Sai Venkatesh Pingali, Shishir P.S. Chundawat, A. Daniel Jones, Paul Langan, Brian H. Davison and Volker Urban7. Isotopic Labeling of Proteins in Halobacterium SalinarumThomas E. Cleveland and Zvi Kelman8. Amino Acid Selective Unlabeling in Protein NMR SpectroscopyChinmayi Prasanna, Abhinav Dubey and Hanudatta S. Atreya

Labeling in Eukarya9. Isotope Labeling of Eukaryotic Membrane Proteins in Yeast for Solid-State NMR Ying Fan, Sanaz Emami, Rachel Munro, Vladimir Ladizhansky and Leonid S. Brown10. Development of Approaches for Deuterium Incorporation in PlantsBarbara R. Evans and Riddhi Shah11. Isotope labeling of proteins in insect cellsLukasz Skora, Binesh Shrestha and Alvar D. Gossert12. Effective Isotope Labeling of Proteins in a Mammalian Expression SystemMallika Sastry, Carole A. Bewley and Peter D. Kwong

In Vitro Labeling13. Escherichia Coli Cell-Free Protein Synthesis and Isotope Labeling of Mammalian ProteinsTakaho Terada and Shigeyuki Yokoyama14. Rapid Biosynthesis of Stable Isotope Labeled Peptides from a Reconstituted in Vitro Translation System for Targeted Proteomics Feng Xian, Shuwei Li and Siqi Liu15. Labeling of Membrane Proteins by Cell-Free ExpressionAisha Laguerre, Frank Löhr, Frank Bernhard and Volker Dötsch16. Selective Amino Acid Segmental Labeling of Multi-Domain ProteinsErich Michel and Frédéric H.-T. Allain17. Labeling Monosaccharides with Stable IsotopesWenhui Zhang, Shikai Zhao and Anthony S. Serianni