Cell Separation Methods and Applications

Diether Recktenwald

Background: historical and useful methods of preselection and preparative scale sorting. Physical methods of separation: new approaches in density gradient separation using colloidal silica; gradients in the processing of human haematopoietic progenitor cells; centrifugal elutriation - a powerful separation technique in cell biology immunology, and haematology. Antibody-based methods: isolation, activation, expansion, and gene transduction of cell-based therapeutics using polystyrene immunoaffinity devices; the CEPRATER SC system - technology, clinical development, and future directions; high density particles - a novel, highly efficient cell separation technology; antibody and complement mediated cell separation. Magnetic methods: magnetic cell sorting with colloidal superparamagnetic particles; immunomagnetic cell separation using antibodies and superparamagnetic microspheres; free flow magnetophoresis - continuous immunomagnetic sorting of cells and organelles by magnetic deviation and focusing. Flow cytometry: cell separation using flow cytometric cell sorting. Special applications: employing surface markers for the selection of transfected cells; CD34 cell; sorting and enrichment - applications in blood banking and transplantation. Appendices: cellular properties for cell separation; CD antigen designations. Glossary.