P-Type ATPases

An Introduction to P-type ATPase research.-  Purification of Na,K-ATPase from Pig Kidney.- Preparation of Ca2+-ATPase1a Enzyme from Rabbit Sarcoplasmic Reticulum.- Isolation of H+/K+-ATPase-enriched Membrane Fraction from Pig Stomachs.- Overproduction of PiB-type ATPases.- Coordinated Overexpression in Yeast of a P4-ATPase and its Associated Cdc50 Subunit: The Case of the Drs2p/Cdc50p Lipid Flippase Complex.- The Plasma Membrane Ca2+-ATPase: Purification by Calmodulin Affinity Chromatography, and Reconstitution of the Purified Protein.- Expression of Na,K-ATPase and H,K-ATPase Isoforms With The Baculovirus Expression System.- Time-dependent Protein Thermostability Assay.- Colorimetric Assays of Na,K-ATPase.- ATPase Activity Measurements by an Enzyme-Coupled Spectrophotometric Assay.- Antimony-Phosphomolybdate ATPase Assay.- ATPase Activity Measurements using radiolabeled ATP.- Assaying P-type ATPases Reconstituted in Liposomes.- Coupling ratio for Ca2+ Transport by Calcium Oxalate Precipitation.- Calcium Uptake in Crude Tissue Preparation.- Measuring H+ Pumping and Membrane Potential Formation in Sealed Membrane Vesicle Systems.- Assay of Flippase Activity in Proteoliposomes using Fluorescent Lipid Derivatives.- The use of Metal Fluoride Compounds as Phosphate Analogs for Understanding the Structural Mechanism in P-type ATPases.- Phosphorylation/Dephosphorylation Assays.- Tryptophan Fluorescence Changes Related to Ca2+-ATPase Function.- Determination of the ATP Affinity of the Sarcoplasmic Reticulum Ca2+-ATPase by Competitive Inhibition of [g-32P]TNP-8N3-ATP Photolabeling.





23.  Ca2+ Binding and Transport Studied with Ca2+/EGTA Buffers and 45Ca2+



24.  Assay of Copper Transfer and Binding to P1B-ATPases



 



25.  Voltage Clamp Fluorometry of P-Type ATPases



26.  Electrophysiological Measurements on Solid Supported Membranes



27.  Electrophysiological Characterization of Na,K-ATPases Expressed in Xenopus laevis Oocytes Using Two-Electrode Voltage Clamping

 

 



28.  Functional Studies of Na+,K+-ATPase using Transfected Cell Cultures



29.  HPLC Neurotransmitter Analysis

 

30.  Behavior Test Relevant to a2/a3Na+/K+-ATPase Gene Modified Mouse Models

 

31.  Zebrafish Whole-mount in situ Hybridization Followed by Sectioning

 

32.  Whole-mount Immunohistochemistry for Anti-F59 in Zebrafish Embryos (1-5 days Post Fertilization (dpf)) Canan Doganli



33.  Cell-based Lipid Flippase Assay Employing Fluorescent Lipid Derivatives

 

34.  Transient Expression of P-type ATPases in Tobacco Epidermal Cells



 



35.  Lipid Exchange by Ultracentrifugation



36.  Reconstitution of Na+, K+-ATPase in Nanodiscs





37.  Crystallization of P-type ATPases by the High Lipid-detergent (HiLiDe) Method



38.  Two-dimensional Crystallization of the Ca2+-ATPase for Electron Crystallography

39.  Two-dimensional  Crystallization  of  Gastric  H+,K+-ATPase  for  Structural  Analysis  By Electron Crystallography





40.  MD Simulations of P-type ATPases in a Lipid Bilayer System

41.  Computational Classification of P-Type ATPases

 

42.  Molecular Modeling of Fluorescent SERCA Biosensors

 

43.  How to Compare, Analyze and Morph Between Crystal Structures of Different Conformations: The P-type ATPase Example

Jesper L