Cytoskeleton Methods and Protocols
Humana Press Inc. (Verlag)
978-1-4939-3123-1 (ISBN)
Authoritative and thorough, Cytoskeleton Methods and Protocols, Third Edition helps researchers expand their understanding of cytoskeleton structure and function.
Long-Term Live Cell Imaging of Cell Migration: Effects of Pathogenic Fungi on Human Epithelial Cell Migration.- Live-Cell Imaging of Mitochondria and the Actin Cytoskeleton in Budding Yeast.- Imaging of the Actin Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells.- Imaging of the Cytoskeleton Using Live and Fixed Drosophila Tissue Culture Cells.- Imaging Cytoskeleton Components by Electron Microscopy.- Purification and Localization of Intraflagellar Transport Particles and Polypeptides.- Fluorescence Imaging of the Cytoskeleton in Plant Roots.- Microtubules in Plant Cells: Strategies and Methods for Immunofluorescence, Transmission Electron Microscopy, and Live Cell Imaging.- Basic Methods to Visualize Actin Filaments In Vitro Using Fluorescence Microscopy for Observation of Filament Severing and Bundling.- An In Vitro Model System to Test Mechano-Microbiological Interactions Between Bacteria and Host Cells.- Reconstitution of a Minimal Actin Cortex by Coupling Actin Filaments to Reconstituted Membranes.- Use of Nanobodies to Localize Endogenous Cytoskeletal Proteins and to Determine Their Contribution to Cancer Cell Invasion by Using an ECM Degradation Assay.- Actin-Dynamics in Plant Cells: The Function of Actin Perturbing Substances: Jasplakinolide, Chondramides, Phalloidin, Cytochalasins, and Latrunculins.- Quantitative Motion Analysis in Two and Three Dimensions.- Measurement of Cell Motility Using Microgrooved Substrates.- The Study of Cell Motility by Cell Traction Force Microscopy (CTFM).- Melanosome Motility in Fish Retinal Pigment Epithelial (RPE) Cells.- Analysis of Stem Cell Motility In Vivo Based on Immunodetection of Planarian Neoblasts and Tracing of BrdU-Labeled Cells After Partial Irradiation.- Chemotaxis: Under Agarose Assay.- Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila.- Proteomic Analysis of Cytoskeleton Proteins in Fish.- Using a Hand-Held Gene Gun forGenetic Transformation of Tetrahymena Thermophila.- Proteomic Tools for the Analysis of Cytoskeleton Proteins.- Homology Modeling Procedures for Cytoskeletal Proteins of Tetrahymena and Other Ciliated Protists.
Reihe/Serie | Methods in Molecular Biology ; 1365 |
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Zusatzinfo | 38 Illustrations, color; 53 Illustrations, black and white; XIII, 431 p. 91 illus., 38 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Schlagworte | Cell and organelle motility • Cell-Imaging • Cytoskeleton Components • cytoskeleton function • Cytoskeleton Structure • In Vitro Reconstructions • Proteomics |
ISBN-10 | 1-4939-3123-7 / 1493931237 |
ISBN-13 | 978-1-4939-3123-1 / 9781493931231 |
Zustand | Neuware |
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