HIV Protocols
Humana Press Inc. (Verlag)
978-1-4939-3045-6 (ISBN)
Authoritative and cutting-edge, HIV Protocols, Third Edition is a great resource for HIV research, and contains many significant tips and tricks rarely mentioned in standard protocols.
Quantifying CD4/CCR5 Usage Efficiency of HIV-1 ENV Using the Affinofile System.- Measuring T Cell-to-T Cell HIV-1 Transfer, Viral Fusion, and Infection Using Flow Cytometry.- HIV-1 Capsid Stabilization Assay.- Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Viral Core Integrity.- HIV-1 Reverse Transcriptase Based Assay to Determine Cellular dNTP Concentrations.- Rapid Determination of HIV-1 Mutant Frequencies and Mutation Spectra Using an mCherry/EGFP Dual Reporter Viral Vector.- Novel Biochemical Tools for Probing HIV RNA Structure.- Analysis of HIV-1 Gag-RNA Interactions in Cells and Virions by CLIP-seq.- Isolation of Cognate Cellular and Viral Ribonucleoprotein Complexes of HIV-1 RNA Applicable to Proteomic Discovery and Molecular Investigations.- Methods for the Analyses of Inhibitor-Induced Aberrant Multimerization of HIV-1 Integrase.- Quantification of HIV-1 Gag Localization Within Virus Producer Cells.- Methods to Study Determinants for Membrane Targeting of HIV-1 Gag In Vitro.- Visualizing the Behavior of HIV-Infected T Cells In Vivo Using Multiphoton Intravital Microscopy.- Modeling HIV-1 Mucosal Transmission and Prevention in Humanized Mice.- High Throughput Humanized Mouse Models for Evaluation of HIV-1 Therapeutics and Pathogenesis.- Measuring the Frequency of Latent HIV-1 in Resting CD4+ T Cells Using a Limiting Dilution Co-Culture Assay.- LGIT In Vitro Latency Model in Primary and T Cell Lines to Test HIV-1 Reactivation Compounds.- Improved Methods to Detect Low Levels of HIV Using Antibody-Based Technologies.- Analysis of ABCA1 and Cholesterol Efflux in HIV-Infected Cells.- The Proteomic Characterization of Plasma or Serum from HIV-Infected Patients.- Proteomic Characterization of Exosomes from HIV-1-Infected Cells.- Detecting HIV-1 Tat in Cell Culture Supernatants by ELISA or Western Blot.- Protocol for Detection of HIV-Tat Protein in Cerebrospinal Fluid by a Sandwich Enzyme Linked Immunosorbent Assay.- Measuring the Uptake and Transactivation Function of HIV-1 Tat Protein in a Trans-Cellular Co-Cultivation Set Up.- Evaluating the Role of Viral Proteins in HIV-Mediated Neurotoxicity Using Primary Human Neuronal Cultures.
Erscheint lt. Verlag | 29.12.2015 |
---|---|
Reihe/Serie | Methods in Molecular Biology ; 1354 |
Zusatzinfo | 36 Illustrations, color; 27 Illustrations, black and white; XIV, 383 p. 63 illus., 36 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin |
Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | HIV-1 latency • HIV-1 replication • HIV-1 RNA structure • NeuroAIDS • RNA protein interactions • Virology |
ISBN-10 | 1-4939-3045-1 / 1493930451 |
ISBN-13 | 978-1-4939-3045-6 / 9781493930456 |
Zustand | Neuware |
Haben Sie eine Frage zum Produkt? |
aus dem Bereich