Methods in Mouse Atherosclerosis

Use of Mouse Models in Atherosclerosis Research.- Cytokines and Immune Responses in Murine Atherosclerosis.- Intravital Microscopy for Atherosclerosis Research.- Non-invasive Molecular Imaging of Mouse Atherosclerosis.- Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root.- Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages.- Isolation of Mouse Primary Aortic Endothelial Cells by Selection with Specific Antibodies.- Isolation and Culture of Aortic Smooth Muscle Cells and In Vitro Calcification Assay.- Immunostaining of Macrophages, Endothelial Cells, and Smooth Muscle Cells in the Atherosclerotic Mouse Aorta.- Immunostaining of Lymphocytes In Mouse Atherosclerotic Plaque.- Flow Cytometric Analysis of Immune Cells Within Murine Aorta.- In Vitro Differentiation of Naïve CD4+ T cells: A Tool for Understanding the Development of Atherosclerosis.- Quantification of Apoptosis in Mouse Atherosclerotic Lesions.- Quantification of Cellular Proliferation in Mouse Atherosclerotic Lesions.- Quantification of in vitro Macrophage Cholesterol Efflux and in vivo Macrophage-Specific Reverse Cholesterol Transport.- In vitro Macrophage Phagocytosis Assay.- Glucose and Insulin Tolerance Tests in the Mouse.- Wire Myography to Study Vascular Tone and Vascular Structure of Isolated Mouse Arteries.- Generation of Aorta Transcript Atlases of Wild-type and Apolipoprotein E-null Mice by Laser Capture Microdissection-based mRNA Expression Microarrays.- Analysis of Gene and Protein Expression In Atherosclerotic Mouse Aorta By Western Blot and Quantitative Real-Time PCR.- Bone Marrow Transplantation in Mice to Study the Role of Hematopoietic Cells in Atherosclerosis.- Tandem Stenosis to Induce Atherosclerotic Plaque Instability in the Mouse.- Detection of Intraplaque Hemorrhage in Mouse Atherosclerotic Lesions.- Mechanical Stabilization ofMouse Carotid Artery for in vivo intravital Microscopy Imaging of Atherogenesis.- Intravital Microscopy in the Cremaster Muscle Microcirculation for Endothelial Dysfunction Studies. - Fluorescent Molecular Tomography for in vivo Imaging of Mouse Atherosclerosis.- In vivo 18F-FDG-PET Imaging in Mouse Atherosclerosis.- Magnetic Resonance Imaging of the Atherosclerotic Mouse Aorta.