Correlation Force Spectroscopy for Single Molecule Measurements (eBook)

Milad Radiom received his PhD in Chemical Engineering at Virginia Tech in 2014, after MEng and BSc in Thermal and Fluids Engineering and Mechanical Engineering respectively from Nanyang Technological University and Amirkabir University of Technology. Thereafter, he was appointed as a postdoctoral research associate in Laboratory of Colloid and Surface Chemistry, University of Geneva.  His research interests are physical chemistry of polymers, colloids and surfaces as related to single molecule force spectroscopy, micro-rheology and surface forces.

Chapter 1 - IntroductionOverview of Single Molecule Force Spectroscopy TechniquesExperiments Using Single molecule AFMLimitations of AFM-based single molecule force spectroscopySummary Chapter 2- Correlation Force Spectroscopy Correlation Force Spectroscopy: Rationale Correlation Force Spectroscopy: DevelopmentLaterally Offset Configuration Vertically Offset Configuration Analysis of Correlations between Two Cantilevers Validation of Fluctuation-Dissipation Theorem for One Cantilever Analysis of Thermal Fluctuations to obtain Correlations Chapter 3- Dynamics of Single Molecules Elastic Properties of Single Molecules: Worm-Like Chain and Freely-Jointed Chain ModelsHydrodynamics of Single Molecules: Dumbbell Model and Rouse ModelInternal FrictionRouse with Internal FrictionModel of Linear Viscoelasticity of a Semiflexible ChainSummaryChapter 4- Microrheology with Correlation Force SpectroscopyExisting Techniques of RheometryExperimental MethodsComparison to Finite Element AnalysisComparison to Simple Harmonic Oscillator ModelSummaryChapter 5- Development of Colloidal Probe Correlation Force Spectroscopy: Case StudyMaterials and MethodsAnalysisResults Discussion Summary Chapter 6- Correlation Force Spectroscopy for Single Molecule MeasurementsEffect of the Distance between Cantilever TipsHarmonic Oscillator Modeling of Vertically Offset Correlation Force SpectroscopySummaryChapter 7- Single Molecule Force Spectroscopy of DextranMaterials and MethodsResultsStretching a Dextran MoleculeStatic Force-Elongation ModeDynamic Correlated Fluctuations ModeDiscussionSummary Chapter 8- Single Molecule Force Spectroscopy of Single-Stranded DNARationale Introduction Materials and Methods Modal AnalysisResults DiscussionSummaryChapter 9- SummaryAn Overview of Chapters Future WorkAppendix I: Simple Harmonic Oscillator Model for Laterally Offset Correlation Force SpectroscopyAppendix II: Simple Harmonic Oscillator Model for Vertically Offset Correlation Force Spectroscopy with Tethered MoleculeReferences