Handbook of Vascular Biology Techniques (eBook)

PrefaceSection 1 In vitro techniquesChapter 1: In vitro angiogenesis assay - endothelial migration, proliferation, and tube formationChapter 2: Endothelial cell tube formation on basement membrane to study cancer neoangiogenesisChapter 3: Induction of hypoxia in vascular endothelial cell cultureChapter 4: Evaluating In Vitro Angiogenesis using Live Cell ImagingChapter 5: Isolation of Endothelial Progenitor Cells (EPCs)Chapter 6: Culture and Maintenance of Human Embryonic Stem Cells: A Potential Source for VasculargenesisChapter 7: Assessment of vascular function and contractility, ex vivo.Chapter 8: Measurement of Aβ uptake by cerebrovascular smooth muscle cellsChapter 9: Measurement of Intracellular Ca2+ in Human Endothelial cells Section 2: In vivo and ex vivo manipulationsChapter 10: Evaluation of angiogenesis and arteriogenesis in a mouse model of prolonged cerebral hypoperfusionChapter 11: Sponge implant model of inflammatory angiogenesisChapter 12: The chick embryo chorioallantoic membrane assayChapter 13: Dorsal air sac assayChapter 14: Scanning Electron Microscopy of Blood Vascular Corrosion Casts (SEM on VCC) in mammalsChapter 15: Hypoxia-induced retinal angiogenesis in adult zebrafishChapter 16: Angiogenesis in regenerating adult zebrafish tail finChapter 17: Methods for studying developmental angiogenesis in zebrafishChapter 18: Isolation and Expansion of Brain Microvascular Endothelial Cells Section 3: Imaging and histological analysisChapter 19: Adipose angiogenesisChapter 20: Assessing tumor angiogenesis in histological samplesChapter 21: Whole-mount immunostaining methods to study the blood and lymphatic vasculature in the  embryonic mouse skin and adult mouse cornea.Chapter 22: Computed Tomography Angiography: Fundamental techniques and data interpretationChapter 23: Magnetic Resonance Angiography: Fundamental Techniques and Data InterpretationChapter 24: Single-Photon Emission Tomography of the brain in vascular pathology Section 4: Miscellaneous novel techniques in vascular biologyChapter 25: Enhancing Endothelialisation of Artificial/Engineered Blood Vessels Using Structural CuesChapter 26: Preparation of liposomes with dual fluorophores to follow real-time content release in vivo Chapter 27: Vascular Flow Modelling using Computational Fluid DynamicsChapter 28: Reverse Transcription Real-Time PCR Protocol for gene expression analysesChapter 29: Oscillations, Feedback and Bifurcations in Mathematical Models of Angiogenesis and HaematopoiesisChapter 30: Genomic microarray analysisChapter 31: Selection of appropriate housekeeping genes for quality controlChapter 32: Endothelial transcriptomic analysisChapter 33: Protocol for Multiplex Amplicon Sequencing using Barcoded PrimersChapter 34: Flow cytometry Enumeration of Hematopoietic & Progenitor Stem Cells:  Identification and QuantificationChapter 35: A Scheme for the Development and Validation of Enzyme Linked Immunosorbent Assays (ELISA) for Measurement of Angiogenic Biomarkers in Human BloodChapter 36: Analysis of Phosphorylated Protein Kinases in Endothelial cells by Flow Cytometry