Haematin Enzymes (eBook)

Front Cover 1
Haematin Enzymes, Part 1 6
Copyright Page 7
Table of Contents 10
PREFACE 8
PARTICIPANTS 20
PRESIDENTIAL ADDRESS 24
CHAPTER 1. THE ELECTRONIC STRUCTURE AND ELECTRON TRANSPORT PROPERTIES OF METAL IONS PARTICULARLY IN PORPHYRIN COMPLEXES 26
INTRODUCTION 26
LIGAND-FIELD THEORY OF REGULAR OCTAHEDRAL COMPLEXES 26
LIGAND-FIELD THEORY OF METAL PORPHYRIN COMPLEXES 31
PARAMAGNETIC RESONANCE EXPERIMENTS 32
ELECTRON-TRANSPORT BETWEEN METAL IONS AND THE UTILIZATION OF THE REDOX EERGY 34
REFERENCES 37
DISCUSSION 38
CHAPTER 2. THE ROLE OF THE METAL IN PORPHYRIN COMPLEXES 44
INTRODUCTION 44
CHARGE DISTRIBUTION IN COMPLEXES 45
OXIDATION STATE OF THE METAL IN COMPLEXES 47
REDOX POTENTIALS OF RUTHENIUM COMPLEXES 49
SUMMARY 51
REFERENCES 51
DISCUSSION 52
CHAPTER 3. THE PHYSICO-CHEMICAL BEHAVIOUR OF PORPHYRINS SOLUBILIZED IN AQUEOUS DETERGENT SOLUTIONS 54
SUMMARY 61
REFERENCES 61
DISCUSSION 62
CHAPTER 4. SOME PHYSICAL PROPERTIES AND CHEMICAL REACTIONS OF IRON COMPLEXES 66
SPECTRA 66
OXIDATION-REDUCTION POTENTIALS 70
CHEMICAL REACTIONS OF IRON COMPLEXES 74
ELECTRON TRANSPORT 76
NOTES 77
SUMMARY 77
REFERENCES 77
DISCUSSION 78
CHAPTER 5. SPECTRA AND REDOX POTENTIALS OF METALLOPORPHYRINS AND HAEMOPROTEINS 81
SOME THEORETICAL ASPECTS OF PORPHYRINS AND METALLOPORPHYRINS 83
THE SPECTRA OF SOME PORPHYRINS AND THEIR METAL COMPLEXES 88
OXIDATION-REDUCTION POTENTIALS 92
SUMMARY AND CONCLUSIONS 94
REFERENCES 95
DISCUSSION 96
CHAPTER 6. MODIFICATION OF THE SECONDARY STRUCTURE OF HAEMOPROTEIN MOLECULES 105
RESULTS AND DISCUSSION 105
SUMMARY 118
REFERENCES 119
DISCUSSION 119
CHAPTER 7. ON THE STABILITY OF OXYHAEMOGLOBIN 123
RESULTS AND DISCUSSION 124
SUMMARY 127
REFERENCES 127
DISCUSSION 127
CHAPTER 8. FERRIHAEMOPROTEIN HYDROXIDES: A CORRELATION BETWEEN MAGNETIC AND SPECTROSCOPIC PROPERTIES 130
INTRODUCTION 130
THE INTERPRETATION OF THE MAGNETIC MOMENTS OF THE HYDROXIDES ACCORDING TO VARIOUS THEORIES OF THE ELECTRONIC STRUCTURE OF CO-ORDINATION COMPLEXES 131
QUALITATIVE CORRELATIONS BETWEEN THE MAGNETIC MOMENTS AND THE SPECTRA OF FERRIHAEMOPROTEIN DERIVATIVES 137
QUANTITATIVE CORRELATION BETWEEN THE MAGNETIC MOMENTS AND THE SPECTRA OF FERRIHAEMOPROTEIN HYDROXIDES 145
THE EFFECT OF TEMPERATURE ON THE SPECTRUM AND ON THE MAGNETIC MOMENT OF FERRIMYOGLOBIN HYDROXIDE 153
THE SPECTRA OF FERRIMYOGLOBIN DERIVATIVES IN HEAVY WATER 157
GENERAL REMARKS 158
REFERENCES 163
DISCUSSION 164
CHAPTER 9. ANALYSIS AND INTERPRETATION OF ABSORPTION SPECTRA OF HAEMIN CHROMOPROTEINS 167
MATERIALS AND METHODS 169
EXPERIMENTAL 174
DISCUSSION 190
SUMMARY 193
REFERENCES 193
DISCUSSION 195
CHAPTER 10. THE HAEM-GLOBIN LINKAGE. 3. THE RELATlONSHIP BETWEEN MOLECULAR STRUCTURE AND PHYSIOLOGICAL ACTIVITY OF HAEMOGLOBINS 198
MATERIALS AND METHODS 200
RESULTS 201
DISCUSSION 208
SUMMARY 212
ADDENDUM 213
REFERENCES 213
DISCUSSION 215
CHAPTER 11. EARLY STAGES IN THE METABOLISM OF IRON 219
EXPERIMENTAL 221
RESULTS 223
DISCUSSION 227
SUMMARY 230
ADDENDUM 230
REFERENCES 230
CHAPTER 12. THE ENZYMIC INCORPORATION OF IRON INTO PROTOPORPHYRIN 232
MATERIALS AND METHODS 232
RESULTS 233
REFERENCES 235
DISCUSSION 236
CHAPTER 13. THE LOCATION OF CYTOCHROMES IN ESCHERICHIA COLI 243
MATERIAL AND METHODS 243
EXPERIMENTAL 244
CONCLUSION 248
SUMMARY 248
REFERENCES 248
DISCUSSION 248
CHAPTER 14. ON THE CYTOCHROMES OF ANAEROBICALLY CULTURED YEAST 250
VARIATIONS OF THE HAEMATIN SPECTRUM OF YEAST CULTURED ANAEROBICALLY, AS A FUNCTION OF ITS GROWTH PHASES 250
INDUCTION BY OXYGEN OF RESPIRATORY CYTOCHROME COMPONENTS IN YEASTS CULTURED ANAEROBICALLY 252
FRACTIONATION OF YEAST CELLS IN THE EXPONENTIAL PHASE OF AEROBIC AND ANAEROBIC GROWTH LOCALIZATION OF HAEMATIN ENZYMES IN THE DIFFERENT FRACTIONS
DISCUSSION AND CONCLUSIONS 256
REFERENCES 257
DISCUSSION 258
CHAPTER 15. IRREVERSIBLE INHIBITION OF CATALASE BY THE 3-AMINO-1: 2: 4-TRIAZOLE GROUP OF INHIBITORS IN THE PRESENCE OF CATALASE DONORS 261
THEORY 262
EXPERIMENTAL 265
RESULTS 265
DISCUSSION 267
SUMMARY 269
REFERENCES 269
CHAPTER 16. CATALASE OXIDATION MECHANISMS 270
INTRODUCTION 270
DISCUSSION 270
SUMMARY 277
REFERENCES 277
DISCUSSION 277
CHAPTER 17. STUDIES ON PROBLEMS OF CYTOCHROME c OXIDASE ASSAY 285
INTRODUCTION 285
METHODS 286
RESULTS 288
DISCUSSION 296
SUMMARY 298
REFERENCES 299
DISCUSSION 300
CHAPTER 18. COMPOSITION OF CYTOCHROME c OXIDASE 306
INTRODUCTION 306
COMPONENTS OF CYTOCHROME C OXIDASE 308
REACTIONS OF CYTOCHROME C OXIDASE 315
SUMMARY 323
REFERENCES 324
DISCUSSION 326
CHAPTER 19. CYTOCHROME OXIDASES OF PSEUDOMONAS AERUGINOSA AND OX-HEART MUSCLE, AND THEIR RELATED RESPIRATORY COMPONENTS 327
DISCUSSION 336
REFERENCES 338
DISCUSSION 338
CHAPTER 20. THE ISOLATION, PURIFICATION AND PROPERTIES OF HAEMIN a 347
PURIFICATION OF HAEMIN a AND PORPHYRIN a 347
EVIDENCE FOR A SINGLE PROSTHETIC GROUP FOR MAMMALIAN CYTOCHROMES a AND a3 350
THE PROSTHETIC GROUP OF CYTOCHROME a1 351
QUANTITATIVE MEASUREMENT OF HAEMIN a AND PROTOHAEMIN IN TISSUES 351
SPECTROSCOPIC PROPERTIES OF HAEM a COMPOUNDS AND HAEMOPROTEINS a 352
SUMMARY 356
ADDENDUM 356
REFERENCES 356
DISCUSSION 357
CHAPTER 21. CYTOCHROME OXIDASE COMPONENTS 362
THE PROSTHETIC GROUP OF CYTOCHROME OXIDASE 362
CYTOCHROMES AND PHOSPHORYLATION 365
REFERENCES 369
CHAPTER 22. THE STRUCTURE OF PORPHYRIN a, CRYPTOPORPHYRIN a AND CHLORIN a2 371
PORPHYRIN A 371
CRYPTOPORPHYRIN A 379
HAEM A2 AND CHLORIN A2 381
SUMMARY 383
ADDENDUM 384
REFERENCES 384
DISCUSSION 385