Cytogenetics of Cells in Culture (eBook)

Front Cover 1
Cytogenetics of Cells in Culture 4
Copyright Page 5
Table of Contents 12
Dedication 6
LIST OF CONTRIBUTORS 8
PREFACE 10
CHAPTER 1. CYTOGENETIC ALTERATIONS IN MIXED CULTURES OF MAMMALIAN SOMATIC CELLS IN VITRO 14
INTRODUCTION 14
CONCLUSIONS AND SUMMARY 21
REFERENCES 23
CHAPTER 2. HYBRIDIZATION OF SOMATIC CELLS IN VITRO 26
INTRODUCTION 26
HYBRIDS OBTAINED UP TO DATE 26
SELECTION AND VIABILITY OF HYBRIDS 29
CHROMOSOME SEGREGATION 30
SUMMARY 37
ACKNOWLEDGMENTS 37
REFERENCES 37
CHAPTER 3. SELECTION PRESSURE IN MAMMALIAN CELL POPULATIONS 40
INTRODUCTION 40
THE CONTRIBUTION OF ERROR 41
NORMAL SOMATIC TISSUES 44
IRRADIATED SOMATIC TISSUES 47
DISCUSSION 52
SUMMARY 57
ACKNOWLEDGMENTS 57
REFERENCES 57
CHAPTER 4. PHASE CINEMATOGRAPHY STUDIES ON THE EFFECTS OF RADIATION AND OF SOME CHEMICALS ON CELLS AND CHROMOSOMES 60
INTRODUCTION 60
CYTOLOGICAL RESPONSES OF VARIOUS CELLULAR ORGANELLES TO CERTAIN CHEMICALS 61
BEHAVIOR OF CHROMOSOMES IN GRASSHOPPER SPERMATOCYTES FOLLOWING ß-IRRADIATTON 66
BEHAVIOR OF THE TELOPHASE CHROMOSOME GROUP IN SILKWORM SPERMATOCYTES FOLLOWING ULTRAVIOLET MICROBEAM IRRADIATION 72
GENERAL SUMMARY 74
ACKNOWLEDGMENTS 74
REFERENCES 75
CHAPTER 5. CELLULAR INTERPRETATION OF ASPECTS OF THE ACUTE MAMMALIAN RADIATION SYNDROME 76
INTRODUCTION 76
DIFFICULTIES IN INTERPRETATION OF THE RADIATION SYNDROME 76
SURVIVAL CURVES FOR MAMMALIAN CELLS IN VITRO 78
INTERPRETATION OF THE ACUTE WHOLE-BODY RADIATION SYNDROME ON THE BASIS OF CELL REPRODUCTIVE KILLING 81
FURTHER EXPERIMENTAL EVIDENCE FOR THIS FORMULATION 82
APPLICATION OF THESE CONSIDERATIONS TO OTHER ASPECTS OF THE WHOLE-BODY RADIATION SYNDROME 86
IDENTIFICATION OF THE SITE WITHIN THE CELL RESPONSIBLE FOR THE LETHAL ACTION 87
SUMMARY 89
REFERENCES 89
CHAPTER 6. RETENTION OF EUPLOIDY AND MUTAGENICITY OF HETEROCHROMATIN IN CULTURE 92
INTRODUCTION 92
SEX HETEROCHROMATIN 95
RETENTION OF EUPLOIDY M VITRO 95
EUPLOID SPONTANEOUS NEOPLASIA 96
INDUCED MALIGNANCIES 97
IN VIVO TRANSPLANTATION OF POLYOMA-TRANSFORMED EUPLOID CELL LINES 98
SPONTANEOUS TUMOR 99
SPECIFICITY OF BUDR FOR SECONDARY CONSTRICTIONS 101
TELOMERIC ASSOCIATION 104
LESION OF SECONDARY CONSTRICTIONS OF X-CHROMOSOMES OF POLYOMA-TRANSFORMED CELLS 104
CHARACTERISTICS OF AN ADENOVIRUS 106
SUMMARY 107
ADDENDUM 108
REFERENCES 108
CHAPTER 7. THE NATURE, ORIGIN, AND GENETIC IMPLICATIONS OF STRUCTURAL ABNORMALITIES OF THE SEX CHROMOSOMES IN MAN 110
INTRODUCTION 110
STRUCTURAL ABNORMALITIES OF THE X-CHROMOSOME 111
ABNORMALITIES OF THE Y-CHROMOSOME 121
SUMMARY 123
ACKNOWLEDGMENT 123
REFERENCES 123
CHAPTER 8. CHROMOSOME STUDIES IN THE GENERAL POPULATION 124
INTRODUCTION 124
COMPOSITION OF THE STUDY POPULATION 125
METHOD 125
GENERAL POPULATION 132
DISCUSSION 133
SUMMARY 133
REFERENCES 134
CHAPTER 9. ALTÉRATIONS CHROMOSOMIQUES DANS DES CELLULES CULTIVÉES EN PRÉSENCE D'EXTRAITS TISSULAIRES HÉTÉROSPÉCIFIQUES 136
INTRODUCTION 136
MATÉRIEL ET TECHNIQUES 137
DISCUSSION 146
RÉSUMÉ 148
SUMMARY 149
BIBLIOGRAPHIE 149
CHAPTER 10. CHROMOSOMAL STUDIES ON HUMAN LEUCOCYTES FOLLOWING TREATMENT WITH RADIOACTIVE IODINE IN VIVO AND IN VITRO 150
INTRODUCTION 150
MATERIALS AND METHODS 151
RESULTS 152
DISCUSSION 162
SUMMARY 165
ACKNOWLEDGMENTS 166
REFERENCES 166
CHAPTER 11. THE LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS 168
INTRODUCTION 168
MATERIALS AND METHODS 169
EXPERIMENTAL RESULTS 169
DISCUSSION 177
SUMMARY 185
REFERENCES 185
CHAPTER 12. REGULATION OF DNA REPLICATION AND VARIEGATION-TYPE POSITION EFFECTS 188
PATTERNS OF ASYNCHRONOUS REPLICATION 188
LATE REPLICATION, HETEROPYCNOSIS, AND GENETIC INACTIVATION 190
TESTS OF THE INACTIVATION HYPOTHESIS 192
INDUCTION OF THE REPRESSED STATE 194
HYPOTHESIS FOR A GENERAL REPRESSOR SYSTEM 195
SUMMARY AND PREDICTIONS 200
ACKNOWLEDGMENT 201
REFERENCES 201
CHAPTER 13. IDENTIFICATION AND CHARACTERIZATION OF HUMAN CHROMOSOMES BY DNA REPLICATION SEQUENCE 204
INTRODUCTION 204
CHROMOSOMAL CHARACTERISTICS 206
COMMENTS 216
SUMMARY 219
REFERENCES 220
CHAPTER 14. MUTAGENIC RESPONSE OF HUMAN SOMATIC CELL LINES 222
INTRODUCTION 222
MATERIALS AND METHODS 223
RESULTS AND DISCUSSION 226
CONCLUSIONS AND SUMMARY 232
ACKNOWLEDGMENTS 233
REFERENCES 233
CHAPTER 15. DNA SYNTHESIS AND CHROMOSOMAL MORPHOLOGY OF CHINESE HAMSTER CELLS CULTURED IN MEDIA CONTAINING W-DEACETYL-N-METHYLCOLCHICINE ( COLCEMID ) 236
INTRODUCTION 236
PROCEDURES 236
EXPERIMENTAL DATA 237
DISCUSSION 257
SUMMARY 260
ACKNOWLEDGMENTS 261
REFERENCES 261
CHAPTER 16. HETEROCHROMATIN IN THE HUMAN MALE 262
INTRODUCTION 262
MATERIALS AND METHODS 262
RESULTS 263
SUMMARY 266
REFERENCES 266
CHAPTER 17. CHROMOSOME BREAKAGE ASSOCIATED WITH VIRUSES AND DNA INHIBITORS 268
INTRODUCTION 268
ROUS SARCOMA 268
MEASLES 270
DEOXYADENOSINE AND CYTOSINE ARABINOSIDE 271
ROUS VIRUS AND HUMAN LEUCOCYTE CULTURES 282
SUMMARY 283
ACKNOWLEDGMENTS 283
REFERENCES 283
CHAPTER 18. QUANTITATION AND AUTOMATION OF CHROMOSOMAL DATA WITH SPECIAL REFERENCE TO THE CHROMOSOMES OF THE HAMSHIRE PIG (SUS SCROFA) 286
INTRODUCTION 286
MATERIALS AND METHODS 286
RESULTS 287
DISCUSSION 309
SUMMARY 318
REFERENCES 318
AUTHOR INDEX 320