RNA Remodeling Proteins

Key Points to Consider When Studying RNA Remodeling by Proteins.- Happy Birthday: 25 years of DEAD-box Proteins.- In vivo Cross-linking Followed by polyA Enrichment to Identify Yeast mRNA Binding Proteins.- Dynamics of the Spb4 Interactome Monitored by Affinity Purification.- Using EMOTE to Map the Exact 5'-ends of Processed RNA on a Transcriptome-wide Scale.- Cellular Localization of RNA Degradation and Processing Components in Escherichia coli.- Recombineering Applications for the Mutational Analysis of Bacterial RNA-binding Proteins and Their Sites of Action.- Determination of RNA Chaperone Activity Using an Escherichia coli Mutant.- Biochemical Characterization of G4 Quadruplex Telomerase RNA Unwinding by the RNA Helicase RHAU.- ATPase Site Configuration of the RNA Helicase DbpA Probed by ENDOR Spectroscopy.- Bioinformatics and Biochemical Methods to Study the Structural and Functional Elements of DEAD-box RNA Helicases.- Measuring Helicase Inhibition of the DEAD-box Protein Dbp2 by Yra1.- A FRET-based, Continuous Assay for the Helicase Activity of DEAD-box Proteins.- A Fluorescence-Based Screening Assay for Identification of Hepatitis C Virus NS3 Helicase Inhibitors and Characterization of Their Inhibitory Mechanism.- Mechanisms of HCV NS3 Helicase Monitored by Optical Tweezers.- Constructing a Magnetic Tweezers to Monitor RNA Translocation at the Single-molecule Level.- Probing RNA Translocases with DNA.- Monitoring RNA Unwinding by the Transcription Termination Factor Rho From Mycobacterium Tuberculosis.- Characterization of the Mechanisms of Transcription Termination by the Helicase Sen1.- Characterization of TRAP-mediated Regulation of the B. subtilis trp Operon Using in vitro Transcription and Transcriptional Reporter Fusions in vivo.- Single-molecule FRET Characterization of RNA Remodeling Induced by an Antitermination Protein.- Fluorescence Reporters for Hfq Oligomerization and RNA Annealing.- Fluorescence Anisotropy-based Salt-titration Approach to Characterize Protein-Nucleic Acid Interactions.- Probing Hfq:RNA Interactions with Hydroxyl Radical and RNase Footprinting.- Purification of Eukaryotic Exoribonucleases Following Heterologous Expression in Bacteria and Analysis of Their Biochemical Properties by in vitro Enzymatic Assays.- Tips and Tricks to Probe the RNA-degrading Activities of Hyperthermophilic Archaeal β-CASP Ribonucleases.