Protocols in Molecular Neurobiology

Separation of Large DNA Molecules by Pulsed-Field Gel Electrophoresis.- The Isolation of Genomic DNA from Invertebrates.- Analysis of Mitochondrial DNA Mutations.- The Design and Use of Oligonucleotides.- The Use of Degenerate Oligonucleotides for Polymerase Chain-Reaction-Based Isolation of Related DNA Sequences.- Isolation of Photoreceptor Cell-Specific MEKA cDNA by Differential Hybridization.- Generation of Isoform-Specific Antisera from Cloned cDNAs.- Determination of Transcriptional Initiation Sites and Their Usage in the Nervous System.- Mapping of Trans-Acting Factor Binding in the Nervous System.- In Situ Hybridization to Brain Tissue Sections Using Labeled Single-Strand Complementary RNA Probes.- In Situ Hybridization Histochemistry Using Alkaline Phosphatase-Labeled Oligodeoxynucleotide Probe.- The Identification of Neuropeptide Gene Regulatory Elements in Transgenic Mice.- Expression of Exogenous Ion Channels and Neurotransmitter Receptors in RNA-Injected Xenopus Oocytes.- Analysis of Insulin and Insulin-Like Growth Factor-I Receptors in Neural Tissues.- Use of Affinity Chromatography in Purification of A1 Adenosine Receptors from Rat Brain Membranes.- Purification and Structure of L-Type Calcium Channels.- Purification and Reconstitution of the Ryanodine-Sensitive Ca2+ Release Channel Complex from Muscle Sarcoplasmic Reticulum.- Identification of a Ligand-Gated Ion Channel by Photoaffinity Labeling and Microsequencing.- Voltage-Gated Ion Channels.- An Electrophysiological Approach to the Regulation of Neuronal Voltage-Activated Calcium Channels by Guanine Nucleotide Binding Proteins.