Animal Cell Electroporation and Electrofusion Protocols

Theory and Instrumentation.- Electroporation Theory.- Effects of Pulse Length and Strength on Electroporation Efficiency.- Instrumentation.- Electroporation Protocols.- The Introduction of Proteins into Mammalian Cells by Electroporation.- Electroporation of Antigen-Presenting Cells for T-Cell Recognition and Cytotoxic T-Lymphocyte Priming.- Electroporation of Antibodies into Mammalian Cells.- Electroporation of Adherent Cells In Situ for the Introduction of Nonpermeant Molecules.- Electrotransformation of Chinese Hamster Ovary Cells.- Electroporation of Rat Pituitary Cells.- Electroporation of Plasmid DNA into Normal Human Fibroblasts.- Electroporation-Mediated Gene Transfer into Hepatocytes.- Electroporation of Human Lymphoblastoid Cells.- The Use of Electroporated Bovine Spermatozoa to Transfer Foreign DNA into Oocytes.- Electroporation of Embryonic Stem Cells for Generating Transgenic Mice and Studying In Vitro Differentiation.- Electrotransfection with “Intracellular” Buffer.- Effect of Cis-Located Human Satellite DNA on Electroporation Efficiency.- Quantitation of Transient Gene Expression.- Stable Integration of Vectors at High Copy Number for High-Level Expression in Animal Cells.- Electroporation of Drosophila Embryos.- Transformation of Fish Cells and Embryos.- Electroporation of Cardiac Cells.- Electroporation for Gene Therapy.- Electrofusion Protocols.- Electrofusion of Mammalian Cells.- Stabilizing Antibody Secretion of Human Epstein Barr Virus-Activated B-Lymphocytes with Hybridoma Formation by Electrofusion.- Electrofusion of Mammalian Oocytes and Embryonic Cells.- Nuclear Transfer in Bovine Embryos.- Electrofusion of Mouse Embryos to Produce Tetraploids.- Spectrofluorometric Assay for Cell-Tissue Electrofusion.- Cytometric Detection and Quantitationof Cell-Cell Electrofusion Products.