Stem Cell Protocols

A Short Primer in Stem Cell Biology.- Measurement of Hematopoietic Stem Cell Proliferation, Self-Renewal and Expansion Potential.- Measuring the Aging Process in Stem Cells.- Measuring the Potency of a Stem Cell Therapeutic.- Culturing Protocols for Human Multipotent Adult Stem Cells.- The Isolation of Murine Bone Marrow Scavenging Sinusoidal Endothelial Cells.- Analysis of Circadian Rhythms in Embryonic Stem Cells.- Measuring Stem Cell Circadian Rhythms.- Cryopreservation of Human Pluripotent Stem Cells: A General Protocol.- Biological Differences Between Native and Cultured Mesenchymal Stem Cells – Implications for Therapies.- The Use of Multiparameter Flow Cytometry and Cell Sorting to Characterize Native Human Bone Marrow Mesenchymal Stem Cells (MSC).- High Yield Recovery of Equine MEsenchymal Stem Cells from Umbilical Cord Matrix/Wharton’s Jelly Using a Semi-Automated Process.- Isolation and Functional Assessment of Cutaneous Stem Cells.- Isolation of Adult Stem Cell Populations from the Human Cornea.- Advanced Imaging and Tissue Engineering of the Human Limbal Epithelial Stem Cell Niche.- Isolation and Characterization of Stem Cells in the Adult Mammalian Ovary.- Clonal Culture of Adult Mouse Lung Epithelial Stem/Progenitor Cells.- Culture and Characterization of Mammary Cancer Stem Cells in Mammospeheres.- Isolation and Culture of Primary Glioblastoma Cells from Human Tumor Specimens.