Nuclear G-Protein Coupled Receptors

Studies of Intracellular Angiotensin II.- Single-Cell Microinjection Coupled to Confocal Microscopy to Characterize Nuclear Membrane Receptors in Freshly Isolated Cardiomyocytes.- Design and Application of Light-Activated Probes for Cellular Signaling.- Using Caged Ligands to Study Intracrine Endothelin Signalling In Intact Cardiac Myocytes.- Quantification of Catecholamine Uptake in Adult Cardiac Myocytes.- Characterization of the Interaction Between the Prostaglandin D2 DP1 Receptor and the Intracellular L-Prostaglandin D Synthase.-  Isolation and Study of Cardiac Nuclei from Canine Myocardium and Adult Ventricular Myocytes.- High Resolution Imaging and Function of Nuclear G Protein-Coupled Receptors (GPCRs).- Biochemical Fractionation of Membrane Receptors in the Nucleus.- Functional G Protein-Coupled Receptors on Nuclei From Brain and Primary Cultured Neurons.- Automated Microscopy of Cardiac Myocyte Hypertrophy: A Case Study on the Role of Intracellular a-Adrenergic Receptors.- Measuring Intra-Nuclear and Nuclear Envelope [Ca2+] vs. Cytosolic [Ca2+].- Assessing GPCR and G Protein Signaling to the Nucleus in Live Cells Using Fluorescent Biosensors.- Tandem Affinity Purification to Identify Cytosolic and Nuclear Gbg-Interacting Proteins.- Examining the Effects of Nuclear GPCRs on Gene Expression Using Isolated Nuclei.- Trafficking and Function of GPCRs in the Endosomal Compartment.